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Oceanobacillus saliphilus sp. nov., Isolated from Saline−Alkali Soil in Heilongjiang Province, China

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Abstract

A novel bacterium, designated strain APA_H-1(4)T, was isolated from the saline−alkaline soil, Zhaodong, Heilongjiang Province, China. Phenotypic and chemotaxonomic analyses, and whole-genome sequencing were used to determine the taxonomic position of the strain. Phylogenetic analysis indicated that the isolate belongs to the genus Oceanobacillus, and showed the highest sequence similarity to O. damuensis KCTC 33146T (98.35%, similarity) and ‘O. massiliensis’ DSM 24644 (98.32%). The average nucleotide identity values between strain APA_H-1(4)T and other members of the genus Oceanobacillus were lower than 82% recommended for distinguishing novel prokaryotic species. The digital DNA−DNA hybridization values of strain APA_H-1(4)T with O. damuensis KCTC 33146T and ‘O. massiliensis’ DSM 24644 were 13.60 and 17.60%, respectively. Cells of strain APA_H-1(4)T were Gram-staining positive, motile, aerobic, spore-forming rods (0.5–0.7 × 1.8–2.6 μm) with flagella. The growth was found to occur optimally at 37 °C. The whole-cell hydrolysate contained meso-diaminopimelic acid as the diagnostic cell wall diamino acid. The main detected polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified polar lipid. The predominant respiratory quinone was identified as menaquinone-7 (MK-7). The major cellular fatty acid (>10%) was anteiso-C15:0. The G + C content of the genomic DNA was determined to be 38.4% based on the draft genome sequence. Based on the comparative analysis of polyphasic taxonomic data, strain APA_H-1(4)T represents a novel species of the genus Oceanobacillus, for which the name Oceanobacillus saliphilus sp. nov. is proposed. The type strain is APA_H-1(4)T (=GDMCC 1.2239T = KCTC 43254T).

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Funding

This research was supported by the National Key Research and Development Program of China (2021YFD1500300) and the National Science and Technology Fundamental Resources Investigation Program of China (2021FY100900). WJL and SW were also supported by Introduction project of high-level talents in Xinjiang Uygur Autonomous Region.

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Yu-Ting OuYang, Meng-Meng Li, Ai-Ping Lv, Lan Liu, Ze-Tao Liu, Jian-Yu Jiao, Wen-Jun Li & Li-Xiang Cao

  2. Heilongjiang Academy of Black Soil Conservation & Utilization/Key Lab of Soil Environment and Plant Nutrition of Heilongjiang Province/Heilongjiang Fertilizer Engineering Research Center, Harbin, 150086, People’s Republic of China

    Pin-Jiao Jin & Shuang Wang

  3. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Wen-Jun Li & Shuang Wang

Authors
  1. Yu-Ting OuYang
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  5. Lan Liu
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Contributions

WJL, SW and LXC designed the research and project outline. SW isolated the bacterium. JYJ and LL performed the genomic data analysis. YTOY, MML, APL and ZTL performed the deposition, physiological and chemotaxonomic experiments. YTOY drafted the manuscript. All authors have read and approved the final version of the manuscript.

Corresponding authors

Correspondence to Wen-Jun Li, Li-Xiang Cao or Shuang Wang.

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OuYang, YT., Li, MM., Lv, AP. et al. Oceanobacillus saliphilus sp. nov., Isolated from Saline−Alkali Soil in Heilongjiang Province, China. Curr Microbiol 79, 301 (2022). https://doi.org/10.1007/s00284-022-02997-0

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