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Complete Genome Sequence of Lactobacillus salivarius AR809, a Probiotic Strain with Oropharyngeal Tract Resistance and Adhesion to the Oral Epithelial Cells

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Abstract

Lactobacillus salivarius AR809 was isolated from a healthy adult oral cavity with multiple probiotic properties, such as high antimicrobial activity, adhesion to the oral epithelium, resistance to acidic pH, bile, lysozyme, and H2O2. In this study, to investigate the genetic basis on probiotic potential and identify the functional genes in the strain, the complete genome of strain AR809 was sequenced by Illumina and PacBio platforms. Then comparative genome analysis on 11 strains of Lactobacillus salivarius was performed. The complete genome of AR809 consisted of a circular 1,747,224 bp chromosome with 33.00% GC content and four circular plasmids [pA (247,948 bp), pB (27,292 bp), pC (3349 bp), and pD (2898 bp), respectively]. From among the 1866 protein-coding genes, 130 carbohydrate metabolism-related genes, 18 bacteriocin biosynthesis-related genes, 74 environmental stress-related genes, and a series of adhesion-related genes were identified via clusters of orthologous genes, Koyto Encyclopedia of Genes and Genomes, and carbohydrate-active enzymes annotation. The comparative genome analysis indicated that genomic homology between AR809 and CICC23174 was the highest. In conclusion, the present work provided valuable insights into the gene’s function prediction and understanding the genetic basis on adapting to host oropharyngeal-gastrointestinal tract in strain AR809.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We are grateful to the Department of Otolaryngology, Tongji Hospital of Tongji University, for its efforts in the preliminary work.

Funding

This work was supported by the Project of Shanghai for Agriculture Advance by Science and Technology (2022-02-08-00-12-F01102), National Science Fund for Distinguished Young Scholars of China (No.32025029), the Shanghai Agriculture Applied Technology Development Program of China (2019-02-08-00-07-F01152).

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  1. Yong Yang and Xin Song have contributed equally to this work.

Authors and Affiliations

  1. University of Shanghai for Science and Technology, Shanghai Engineering Research Center of Food Microbiology, Shanghai, 200093, China

    Yong Yang, Xin Song, Zhiqiang Xiong, Yongjun Xia, Guangqiang Wang & Lianzhong Ai

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Contributions

YY and XS contributed equally to this work. YY and XS conceived and wrote the draft manuscript. ZX, YX, and GW edited the manuscript. LA supervised the project.

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Correspondence to Lianzhong Ai.

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This study was approved by the Ethics Committee of Tongji Hospital (KYSB-2016-97).

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This study involved oral sampling from healthy individual. Participants provided their verbal informed consent for oral environment sampling. And all the authors read and approved the final manuscript.

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Yang, Y., Song, X., Xiong, Z. et al. Complete Genome Sequence of Lactobacillus salivarius AR809, a Probiotic Strain with Oropharyngeal Tract Resistance and Adhesion to the Oral Epithelial Cells. Curr Microbiol 79, 280 (2022). https://doi.org/10.1007/s00284-022-02963-w

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