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Identification of Fusarium andiyazi Associated with the Bakanae Disease of Rice in Turkey

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Abstract

Rice is an important nutrient staple for much of the Turkish population. In this study, field surveys were carried out to collect isolates of Fusarium andiyazi, a member of the Fusarium fujikuroi species complex (FFSC) associated with Bakanae disease. As part of the study, morphological and microscopic features of Fusarium andiyazi isolates recovered from infected rice plants were determined. The molecular detection of F. andiyazi was carried out through PCR analysis of partial translation elongation factor 1-alpha (TEF-1α) gene and the internal transcribed spacer (ITS) region. Gib2 and Fgc primers were used for specific detection of strain of FFSC. In the phylogenetic analysis, using the sequences of the partial TEF-1α gene, F. andiyazi were clustered in the same branch with the reference isolates. The pathogenicity tests revealed that the isolates of F. andiyazi were pathogen on susceptible Baldo cultivar under room climate conditions. The disease severity ranged from 3.27% to 86.30%. The pathogen causes typical disease symptoms associated with Bakanae and mostly inhibited seed germination, while most of the isolates caused abnormal elongation compared to the control plants. This is the first report of presence of F. andiyazi causing Bakanae disease on rice in Turkey.

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All data during this study were included in this article.

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Acknowledgements

This work is a part of PhD study. The authors acknowledge the Ministry of Agriculture and Forestry for kindly supporting the project and also thankful to Directorate of Plant Protection Research Institute for technical support.

Funding

This study was supported by Ministry of Agriculture and Forestry, Republic of Turkey (TAGEM-BS-15/12-04/02-09).

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Authors and Affiliations

  1. Directorate of Plant Protection Research Institute-Bornova, İzmir, Turkey

    Yeşim Eğerci

  2. Faculty of Agriculture, Department of Plant Protection, Ege University, İzmir, Turkey

    Pervin Kınay Teksür

  3. General Directorate of Agricultural Research and Policies, Ankara, Turkey

    Ayşe Uysal Morca

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  1. Yeşim Eğerci
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  2. Pervin Kınay Teksür
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Contributions

Conception and design and critical revision of the manuscript for important intellectual content: YE and AUM. Data acquisition and interpretation: YE, PKT, and AUM. Coordination of the research project: PKT. Technical and material support: YE, PKT, and AUM. All authors read and approved the final manuscript.

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Correspondence to Yeşim Eğerci.

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Eğerci, Y., Teksür, P.K. & Morca, A.U. Identification of Fusarium andiyazi Associated with the Bakanae Disease of Rice in Turkey. Curr Microbiol 79, 291 (2022). https://doi.org/10.1007/s00284-022-02962-x

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