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Bio-preservation Effect of Probiotic Lactiplantibacillus plantarum S61 Against Rhodotorula glutinis and Listeria monocytogenes in Poultry Meat

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Abstract

The objective of this work is the study of the antifungal and antibacterial activity of Lactiplantibacillus plantarum S61 strains, isolated from traditional fermenting green olives against Rhodotorula glutinis UMP 22 and Listeria monocytogenes ATCC 19117, and its application in meat as bio-preservative agent. The cell-free supernatant (CFS) of Lpb. plantarum S61 shows high inhibition zones, which are 22.45 ± 0.49 and 17.75 ± 0.35 mm, against Rhodotorula glutinis and Listeria monocytogenes. The minimum fungicidal and bactericidal concentrations of the CFS obtained are 8% (v/v) and 10% (v/v), respectively. The competition assay, realized in liquid medium by co-culture of Lpb. plantarum S61 with Rho Rhodotorula glutinis and L. monocytogenes, led to inhibition percentages of 77.72% and 89.52%, respectively. However, the antimicrobial activity of Lpb. plantarum S61 was revealed a proteinaceous nature. Lpb. plantarum S61 strain allowed the reduction of L. monocytogenes in minced poultry meat during 7 days of storage at 4 °C. In addition, Lpb. plantarum S61 improved the physicochemical and color parameters of poultry minced meat. Lpb. plantarum S61 and/or its antimicrobial compounds can be applied as bio-preservative agent in meat product and food industry.

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Acknowledgements

The authors are grateful to the CNRST (PPR/19/2015), and Tunisian cooperation (17TM06) for their support.

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Authors and Affiliations

  1. Laboratory of Bioressource, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed Premier University, 60 000, Oujda, Morocco

    Houssam Abouloifa, Ismail Hasnaoui, Yahya Rokni, Sara Gaamouche, Reda Bellaouchi, Nabil Ghabbour, Ennouamane Saalaoui & Abdeslam Asehraou

  2. Research Unit of Microbiology, Biomolecules and Biotechnology, Laboratory of Chemistry-Physics and Biotechnology of Molecules and Materials, Faculty of Sciences and Techniques, Mohammedia, Hassan II University of Casablanca, Mohammédia, Morocco

    Houssam Abouloifa

  3. Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, BP: 1177, 3018, Sfax, Tunisia

    Sirine Ben Slima, Imen Trabelsi & Riadh Ben Salah

  4. Research Unit Bioprocess and Biointerfaces, Laboratory of Industrial Engineering and Surface Engineering, National School of Applied Sciences, Sultan Moulay Slimane University, Mghila, 23000, Beni Mellal, Morocco

    Yahya Rokni

  5. Laboratory of Natural Resources and Environment, Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University, B. P 1223, Taza, Morocco

    Nabil Ghabbour

  6. Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road Sidi Mansour 6 km, P.O. Box 1177, 3018, Sfax, Tunisia

    Bassem Jaouadi

Authors
  1. Houssam Abouloifa
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  2. Ismail Hasnaoui
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  10. Bassem Jaouadi
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Contributions

HA: Conceptualization, Methodology, Formal analysis, Data curation, Writing-original draft. IH, SBS, YR, SG, IT and RB: Methodology, Formal analysis, Data curation, Formal analysis. NG, RBS and ES: Writing—review & editing. BJ: Methodology, Formal analysis, AA: Conceptualization, Writing- review & editing, and Supervision. All authors read and approved the manuscript.

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Correspondence to Houssam Abouloifa.

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Abouloifa, H., Hasnaoui, I., Ben Slima, S. et al. Bio-preservation Effect of Probiotic Lactiplantibacillus plantarum S61 Against Rhodotorula glutinis and Listeria monocytogenes in Poultry Meat. Curr Microbiol 79, 232 (2022). https://doi.org/10.1007/s00284-022-02923-4

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