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Isolation and Complete Sequence of One Novel Marine Bacteriophage PHS21 Infecting Pseudoalteromonas marina

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Abstract

PHS21 against Pseudoalteromonas is isolated from Qingdao offshore seawater. The phage was characterized and identified by morphological examination, stability, whole genome sequencing, and bioinformatics analysis. Morphological analysis of PHS21 by transmission electron microscopy shows that belonged to the Siphoviridae family. PHS21 showed strong tolerance with a wide range of temperatures and pH. One-step growth assay indicates that the latent period is about 48 min and the burst size is approximately 218 PFU/cell (plaque forming unit/cell). Its complete genomic sequence is 35,802-bp long with 50 putative open reading frames. Phage PHS21 and PHS3 displayed a very close evolutionary relationship; however, having different DNA packaging proteins indicates that they may have already evolved distinct ways to package DNA in host cells. This study provides the detailed description and genomic characterization of a novel Pseudoalteromonas phage.

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Data Availability

The complete genome sequence with annotation of phage PHS21 is available in the GenBank database under accession number KY379511.2.

Code Availability

Not applicable.

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Acknowledgements

This work was supported by the Natural Science Foundation of China (Nos. 41976117, 42120104006, 42176111, 41806131, 41676178), Fundamental Research Funds for the Central Universities (No. 202172002).

Funding

This work was supported by the Natural Science Foundation of China (Nos. 41976117, 42120104006, 42176111, 41806131, 41676178), Fundamental Research Funds for the Central Universities (No. 202172002).

Author information

Author notes

  1. Yan Li and Huifang Li have contributed equally to this study.

Authors and Affiliations

  1. College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, People’s Republic of China

    Yan Li, Yong Jiang, Hongbing Shao, Hui He & Min Wang

  2. The affiliated hospital of Qingdao University, Qingdao, People’s Republic of China

    Min Wang

  3. UMT-OUC Joint Academic Centre for Marine Studies, Qingdao, People’s Republic of China

    Min Wang

  4. State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University (Xiang’an), Xiamen, People’s Republic of China

    Huifang Li

  5. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, People’s Republic of China

    Huifang Li

Authors
  1. Yan Li
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  2. Huifang Li
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  3. Yong Jiang
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  4. Hongbing Shao
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  5. Hui He
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  6. Min Wang
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Contributions

MW conceived, organized, and supervised the project. YJ, HH contributed to the experiment design and manuscript refinement. YL collected the data and wrote the first draft of the manuscript. HL performed the isolation and characterization experiments. HS conducted the sequencing experiments. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Hui He or Min Wang.

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Li, Y., Li, H., Jiang, Y. et al. Isolation and Complete Sequence of One Novel Marine Bacteriophage PHS21 Infecting Pseudoalteromonas marina. Curr Microbiol 79, 213 (2022). https://doi.org/10.1007/s00284-022-02886-6

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