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Transcriptome Analysis Reveals that MAPK Signaling Pathway Mediates Salt Tolerance of YMR253C ORF in Saccharomyces cerevisiae

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Abstract

The YMR253C open reading frame encodes a membrane protein that is highly expressed in NaCl-resistant Saccharomyces cerevisiae mutants. Whether it mediates NaCl tolerance is unclear. By knocking out YMR253C in S. cerevisiae, we found that the salt tolerance of yeast was reduced, the integrity of the cell wall was impaired, and cell death was induced; transcriptome analysis further revealed that YMR253C gene knockout mediates significant changes of 1291 genes, and YMR253C mediates the regulation of MAPK signal pathways. Therefore, the transmembrane protein YMR253C may regulate the MAPK signaling pathway to regulate the salt stress of S. cerevisiae.

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Acknowledgements

This study was mainly funded by the National Natural Science Foundation of China and the National Natural Science Foundation of Yunnan Province.

Funding

Supported by Grants from the National Natural Science Foundation of China (NSFC; Grant No. 81560502, 81960354), the National Natural Science Foundation of Yunnan Province (Grant No. 2019FE001-094, 202101AY070001-012), the Talent Project of Yunnan Province (2019HB024). The authors acknowledge the editors and reviewers for their positive and constructive comments and suggestions on our study.

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  1. Yu Zhang and Mengyan Li have contributed equally to this work and should be considered joint first authors.

Authors and Affiliations

  1. School of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming, 650500, Yunnan, China

    Yu Zhang & Jiaqing Ma

  2. Science and Technology Achievement Incubation Center, Kunming Medical University, 1168 West Chunrong Road, Yuhua Avenue, Chenggong District, Kunming, 650500, Yunnan, China

    Yu Zhang, Mengyan Li, Juqing Deng, Chunling Bai & Lechun Lyu

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  1. Yu Zhang
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  2. Mengyan Li
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  3. Juqing Deng
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  5. Jiaqing Ma
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  6. Lechun Lyu
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Contributions

LL and JM conceived and designed the research. YZ and ML conducted the experiments and analyzed the data. JD and CB analyzed the data.

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Correspondence to Jiaqing Ma or Lechun Lyu.

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Zhang, Y., Li, M., Deng, J. et al. Transcriptome Analysis Reveals that MAPK Signaling Pathway Mediates Salt Tolerance of YMR253C ORF in Saccharomyces cerevisiae. Curr Microbiol 79, 126 (2022). https://doi.org/10.1007/s00284-022-02818-4

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