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Rummeliibacillus suwonensis: First Time Isolation from Human Feces by Culturomics

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Abstract

Gut microbiota is a complex ecosystem composed by trillions of microorganisms that are crucial for human health or disease status. Currently, there are two methodological options to explore its complexity: metagenomics and culturomics. Culturomics is an approach that uses multiple culture conditions (days of incubation, enrichment factors and growth temperature) and MALDI-TOF mass spectrometry for the identification of bacterial species and sequencing when this method fails. In this paper, we describe how Colturomic’s protocol has allowed the first isolation in human sample of Rummeliibacillus suwonensis, a Gram positive, facultative anaerobe bacterium. The bacterium was isolated from feces of a 69 years old male with amyotrophic lateral sclerosis (ALS) recruited for a clinical trial assessing safety and efficacy of fecal microbiota transplantation in ALS. The first isolation of the microorganism dates back to 2013 from the soil of a South Korean mountain area. In this report, morphological description, biochemical characterization and antibiotic susceptibility tests were performed to outline the bacterial properties.

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Funding

This research was supported by the Italian Ministry of Health, Grant-No. RF-2016–02361616.

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Authors and Affiliations

  1. Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy

    Gianluca Quaranta, Alessandra Guarnaccia, Giovanni Fancello, Maurizio Sanguinetti & Luca Masucci

  2. Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, Rome, Italy

    Maurizio Sanguinetti & Luca Masucci

  3. Internal Medicine, Gastroenterology and Liver Unit, Gastroenterology Area, Fondazione Policlinico Universitario Gemelli, Università Cattolica del Sacro Cuore, Rome, Italy

    Stefano Bibbò & Giovanni Cammarota

  4. Department of Biomedical, Metabolic and Neural Sciences, Center for Neuroscience and Neurotechnology (CfNN), University of Modena and Reggio Emilia, 41125, Modena, Italy

    Jessica Mandrioli & Cecilia Simonini

  5. Neurology Unit, Azienda Ospedaliero Universitaria di Modena, 41125, Modena, Italy

    Jessica Mandrioli & Cecilia Simonini

  6. Department of Clinical and Experimental Medicine, University of Florence, 50134, Florence, Italy

    Amedeo Amedei, Elena Niccolai & Giulia Nannini

  7. SOD of Interdisciplinary Internal Medicine, Azienda Ospedaliera Universitaria Careggi (AOUC), 50134, Florence, Italy

    Amedeo Amedei

Authors
  1. Gianluca Quaranta
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  2. Jessica Mandrioli
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  3. Stefano Bibbò
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  4. Alessandra Guarnaccia
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  5. Giovanni Fancello
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  6. Cecilia Simonini
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  7. Amedeo Amedei
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  8. Elena Niccolai
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  9. Giulia Nannini
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  10. Giovanni Cammarota
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  11. Maurizio Sanguinetti
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  12. Luca Masucci
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Contributions

GQ, AG and LM contributed to the study conception, design and writing. GF and SB performed material preparation and data collection. JM, CS, GC and MS contributed in patient’s recruiting. AA, EN and GN contributed in laboratory activities. LM was the supervisor in writing and corrections.

Corresponding author

Correspondence to Alessandra Guarnaccia.

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Conflict of interest

The authors declare no competing interest.

Ethical Approval

Comitato etico dell’Area Vasta Emilia Nord. Delibera AUSL.RE 2017/0373 28/12/2017. Prot. N. 0,033,775/19-Del 30/07/2019.

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Quaranta, G., Mandrioli, J., Bibbò, S. et al. Rummeliibacillus suwonensis: First Time Isolation from Human Feces by Culturomics. Curr Microbiol 79, 197 (2022). https://doi.org/10.1007/s00284-022-02806-8

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  • DOI: https://doi.org/10.1007/s00284-022-02806-8

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