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The Impact of Fast Microbiology in Intensive Care Units in the Era of Antibiotic Resistance: An Observational Retrospective Study

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Abstract

The increasing prevalence of multi-drug-resistant bacteria responsible for bloodstream infections (BSIs) makes therapeutic choices progressively more complex. Fast microbiology quickly detects the presence of pathogens and clinically relevant determinants of antibiotic resistance, offering the potential for early administration of antibiotics. In this retrospective observational study, we comparatively evaluated the performances of FilmArray and the current standard method using blood samples collected from intensive care unit (ICU) patients with suspected BSI. A full agreement with the standard was observed in 97/102 samples (95.1 ± 4.2%), a mismatch in 3/102 samples (2.9 ± 3.2%) and detection failure in 2/102 cases (1.96 ± 2.7%). Statistical analysis demonstrated a near-perfect/perfect level of agreement between the two methods, with an overall degree of agreement of 95%. The high performance demonstrated by the FilmArray could allow a “watch and wait” approach helping clinicians in decision-making processes related to choice and initiation of the antimicrobial therapy, thus avoiding ineffective and excessive use of drugs.

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Authors and Affiliations

  1. Department of Health Sciences, Section of Anaesthesiology, Intensive Care and Pain Medicine, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy

    Mirko Muzzi, Sergio Fabbri, Caterina Scirè-Calabrisotto, Gianluca Villa & Stefano Romagnoli

  2. Department of Anaesthesia and Intensive Care, Section of Neuroanaesthesia and Reanimation, Azienda Ospedaliero Universitaria Careggi, Largo Brambilla 3, 50139, Florence, Italy

    Bruno Viaggi

  3. Department of Anaesthesia and Intensive Care, Azienda Ospedaliero-Universitaria Careggi, Largo Brambilla 3, 50139, Florence, Italy

    Lorenzo Bechi, Gianluca Villa & Stefano Romagnoli

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  1. Mirko Muzzi
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  2. Bruno Viaggi
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  3. Sergio Fabbri
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  4. Lorenzo Bechi
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  5. Caterina Scirè-Calabrisotto
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  7. Stefano Romagnoli
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Contributions

M.M., B.V., G.V. and S.R. were involved in the conception and design of the study. M.M. and L.B. were involved in the analysis and interpretation of the data. M.M., S.F. and C.S-C. were involved in the drafting of the paper. B.V., G.V. and S.R. were involved in revising the critically for intellectual content. Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Correspondence to Mirko Muzzi.

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Muzzi, M., Viaggi, B., Fabbri, S. et al. The Impact of Fast Microbiology in Intensive Care Units in the Era of Antibiotic Resistance: An Observational Retrospective Study. Curr Microbiol 79, 79 (2022). https://doi.org/10.1007/s00284-022-02773-0

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  • DOI: https://doi.org/10.1007/s00284-022-02773-0

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