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Co-cultivation Approach to Decipher the Influence of Nitrogen-Fixing Cyanobacterium on Growth and N Uptake in Rice Crop

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Abstract

The present study was performed to evaluate the efficacy of selected potential nitrogen-fixing cyanobacterial strain (Anabaena sp.), isolated from rhizospheric soil of rice plants on growth, pigments, N uptake, root architecture, and image-based phenotypic traits of rice crop using co-cultivation approach under controlled sand culture conditions. We studied the beneficial interaction of cyanobacterium to rice using sensor image-based Phenomics approach as well as conventional methods. Co-cultivation experiment revealed that inoculation with Anabaena sp. significantly improved plant growth, chlorophyll, leaf area, % nitrogen, and protein of rice by ~ 70%, ~ 22%, ~ 60%, and ~ 25% under 100% nitrogen input in comparison with un-inoculated control. Further, comparative evaluation revealed superior performance of Anabaena sp. at 100% and 75% N followed by 50% N input improving below-ground parameters as well as phenotypic traits as compared to control treatment. Hence, inoculation performed better with inorganic nitrogen input for overall growth of rice crop. Therefore, cyanobacterial strain can be used as an efficient bio-inoculant for sustainable rice production under integrated nutrient management.

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Acknowledgements

The authors thank Indian Council of Agricultural Research (ICAR)—IARI, New Delhi, for permitting the first author to undertake the present study under PAT (Professional Attachment Training). The authors gratefully acknowledge CCUBGA, Division of Microbiology, National Phytotron Facility, and Phenomics facility of ICAR-IARI, for their help in facilitating the experiments.

Funding

The work is a part of PAT (Professional Attachment Training) undertaken at ICAR (Indian Council of Agricultural Research) and fund and facility provided by Director, IARI (Indian Agricultural Research Institute), New Delhi.

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Authors and Affiliations

  1. ICAR-Indian Agricultural Research Institute, Gauria Karma, Hazaribagh, Jharkhand, 825405, India

    Himani Priya & Ranjit Singh

  2. School of Agricultural Sciences, Sharda University, Knowledge Park III, Greater Noida, 201306, India

    Dolly W. Dhar

  3. Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Sudhir Kumar, Raju Dhandapani & Renu Pandey

  4. Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Venkadasamy Govindasamy

  5. National Phytotron Facility, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Arun Kumar

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  1. Himani Priya
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Contributions

HP planned the experiment, performed bench work, and wrote the draft of the manuscript. SK and RD helped to perform Phenomics observation. RP provided the composition of Modified ‘PuRicH’ (Pusa Rice Hydroponics) medium. RS analyzed and interpreted the data and plotted the figures. VG helped in root scanning observation in the manuscript. AK assisted providing Phytotron Facility. DWD critically revised, guided, improved, and finalized the manuscript to be published.

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Correspondence to Himani Priya.

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Priya, H., Dhar, D.W., Singh, R. et al. Co-cultivation Approach to Decipher the Influence of Nitrogen-Fixing Cyanobacterium on Growth and N Uptake in Rice Crop. Curr Microbiol 79, 53 (2022). https://doi.org/10.1007/s00284-021-02732-1

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