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Veronia nyctiphanis gen. nov., sp. nov., Isolated from the Stomach of the Euphausiid Nyctiphanes simplex (Hansen, 1911) in the Gulf of California, and Reclassification of Enterovibrio pacificus as Veronia pacifica comb. nov.

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Abstract

The bacterial strain 42Xb2 T was isolated from a female adult krill Nyctiphanes simplex infected with the apostome parasitoid ciliate Pseudocollinia brintoni in January 2007 in the Gulf of California. The strain has the morphological, phenotypic, and molecular characteristics of the bacteria of the family Vibrionaceae. The 16S rRNA gene sequence has a similarity of 97.7% with Enterovibrio pacificus SW014 T and 96.1% similarity with Enterovibrio norvegicus LMG 19839 T. A phylogenomic and a multilocus sequence analyses placed this strain close to the genera Enterovibrio, Grimontia, and Salinivibrio, but clearly forming a separate branch from these bacterial genera. Genomic analyses presented further support this result. A novel genus Veronia gen. nov. and a species Veronia nyctiphanis sp. nov. is here described with CAIM 600 T (= DSM 24592 T = CECT 7578 T) as the type strain. Morphological, physiological, and genetic evidence presented here support the unification of Enterovibrio pacificus and Veronia nyctiphanis in the new genus Veronia. Enterovibrio pacificus is reclassified as Veronia pacifica. V. pacifica is assigned as the type species of the new genus Veronia.

Genome Sequencing Data The GenBank/EMBL/DDBJ accession numbers for the genome sequence of Veronia nyctiphanis CAIM 600 T is PEIB01 and of Enterovibrio pacificus CAIM 1920 T is LYBM01. The 16S rRNA gene sequence of V. nyctiphanis CAIM 600 T is JX129353.

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Acknowledgements

We thank Dr. Carlos J. Robinson and the crew of the R/V El Puma and graduate students and researchers at the Fisheries Ecology Laboratory of ICMyL-UNAM, UABCS, and CICIMAR-IPN for the available ship time and their assistance with zooplankton sampling and oceanographic data records. We are grateful to G. Poetter for excellent technical assistance.

Funding

This project was funded by grants from the CICIMAR-IPN (SIP 2007–2013), CONACYT-SEP (132328), CONACYT-FOSEMARNAT (2004-C01-144, 2012-178615-C01, 2016-01-284201), CONACYT-SAGARPA (2005-1-11717), and ICMYL-UNAM (PAPIIT No. IN200610, IN218106-3, and IN2006-10). JGG is EDI-IPN and COFAA-IPN fellow. MJAM and ALC research were supported by CIBNOR PC4.2, SEP-CONACYT 45914/A-1 grants.

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Author notes

  1. Mario J. Aguilar-Méndez

    Present address: Instituto Politécnico Nacional (IPN), Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato (UPIIG), 36275, Silao de la Victoria, Guanajuato, Mexico

Authors and Affiliations

  1. CIAD, A.C. Mazatlán Unit for Aquaculture and Environmental Management, A.P. 711, 82000, Mazatlán, Sinaloa, Mexico

    Bruno Gomez-Gil & Julissa Enciso-Ibarra

  2. Instituto de Ciencias del Mar Y Limnología, Universidad Nacional Autónoma de México (Unidad Académica Mazatlán), 82000, Mazatlán, México

    Adrián González-Castillo

  3. Laboratorio de Geomicrobiología Y Biotecnología, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), 23096, La Paz, BCS, Mexico

    Mario J. Aguilar-Méndez & Alejandro López-Cortés

  4. Instituto Politécnico, Nacional, Centro Interdisciplinario de Ciencias Marinas, Av. IPN s/n, Col. Palo de Santa Rita, 23096, La Paz, BCS, Mexico

    Jaime Gómez-Gutiérrez

  5. Institut de Recerca I Tecnologia Agroalimentaries, Centre d’Aquicultura, Sant Carles de La Rapita, Spain

    Ana Roque

  6. Leibniz Institute DSMZ - Deutsche Sammlung Von Mikroorganismen Und Zellkulturen GmbH, Inhoffenstr. 7B, 38124, Braunschweig, Germany

    Elke Lang

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Contributions

All authors contributed to the study conception and design. BGG, AGC, and JEI performed material preparation, data collection and analysis. Conceptualization was performed by MJAM; Visualization was performed by ALC; Data Curation was performed by JGG; Methodology and Validation were performed by AR and EL. BGG wrote the first draft of the manuscript and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Bruno Gomez-Gil.

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Gomez-Gil, B., González-Castillo, A., Aguilar-Méndez, M.J. et al. Veronia nyctiphanis gen. nov., sp. nov., Isolated from the Stomach of the Euphausiid Nyctiphanes simplex (Hansen, 1911) in the Gulf of California, and Reclassification of Enterovibrio pacificus as Veronia pacifica comb. nov.. Curr Microbiol 78, 3782–3790 (2021). https://doi.org/10.1007/s00284-021-02627-1

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