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Isolation, Identification and Characterization of Paenibacillus pabuli E1 to Explore Its Aflatoxin B1 Degradation Potential

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Abstract

Aflatoxin B1 (AFB1) contamination in feed and food seriously threatens the healthy growth of animals and humans, and it may lead to huge economic losses in livestock and poultry production. Therefore, screening of high-efficient AFB1-degrading bacteria is necessary to ensure the safety of feed and food. The study aims to isolate and characterize bacteria from various sources to explore its AFB1 degradation potential. Fifteen bacterial were obtained using a medium containing coumarin as the sole carbon source; only one strain showed a good-degrading ability in culture media by adding AFB1 and it was selected for further studies. A gram-negative and spore-forming, designated E1, was identified as Paenibacillus pabuli, with the highest sequence similarity to P. pabuli NBRC13638T (98.97%). The growth of the strain E1 was observed under 22–47 °C, pH 5.5–9.5 and NaCl concentration 0–6% (w/v), with optimum growth at 37 °C, pH 7.5 and 1% NaCl. The biodegradation characteristics of object strain were detected by high performance liquid chromatography (HPLC). The degradation ratio of AFB1 reached 55% at 24 h and 70.2% at 48 h. After 96 h, the degradation rate of AFB1 reached 85.9%. The active degradation components were present in the cell-free supernatant of strain E1, and the degradation ratio of AFB1 reached 80.0% after 96 h. It is the first report that genus Paenibacillus could degrade AFB1. Moreover, E1 has highly adaptable to diverse environmental conditions. It will be a potential candidate for biodegradation of mycotoxins in feed and food.

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Acknowledgements

The authors are grateful to the State Key Laboratory of Fine Chemicals for their excellent service in performing electron microscopy and HPLC analyses for this study, respectively.

Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 41861124004) and the Open Research Fund Program of Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry (CP-2019-YB2).

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Authors and Affiliations

  1. School of Bioengineering, Dalian University of Technology, Dalian, 116024, China

    Gen Li, Xiaoyu Li, Panpan Zou, Bilal Murtaza, Bowen Jin, Hong Zhao, Lili Wang & Yongping Xu

  2. Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China

    Liming Dong

  3. Animal Husbandry Working Station of Ulanhot City, Hinggan League, 137400, China

    Caixia Li

  4. Department of Pathology, University of Agriculture, Faisalabad, 38000, Pakistan

    Muhammas Kashif Saleemi

  5. Dalian SEM Bio-Engineering Technology Co. Ltd., Dalian, 116620, China

    Shuying Li, Huan Yang & Yongping Xu

  6. Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China

    Yongping Xu

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  1. Gen Li
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Contributions

GL contributed to performing the experiments, analyzing data, and writing the initial draft. CL, BJ, HZ and HY collected, processed and classified all samples. GL and PPZ performed isolation and identification of AFB1-degrading strain, and explored AFB1 degradation characteristics. YX, SL designed and supervised the study, and edited the original draft. BM and MKS performed the instrument and revised of the language of article. The project fund management, and final manuscript preparation were performed by YX, XL, LD, and LW. All authors read and approved the manuscript.

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Correspondence to Yongping Xu.

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Li, G., Li, X., Dong, L. et al. Isolation, Identification and Characterization of Paenibacillus pabuli E1 to Explore Its Aflatoxin B1 Degradation Potential. Curr Microbiol 78, 3686–3695 (2021). https://doi.org/10.1007/s00284-021-02624-4

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