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Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica

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Abstract

During our studies focused on the microorganism diversity and community structure of Populus euphratica at Ebinur lake wetland nature reserve in Xinjiang Uyghur Autonomous Region, PR China, a Gram-positive, aerobic, short rod-shaped bacterium without flagellum, designated as MD2T, was isolated from a piece of resina on Populus euphratica. The isolate grew at temperature of 10–45 °C (optimum 37 °C), pH of 7.0–12.0 (optimum pH 8.0) and NaCl concentration of 1–18% (optimum 3%, w/v). Based on the 16S rRNA gene sequences and the phylogenetic analysis, the strain shared the highest sequence similarities to Nesterenkonia alkaliphila JCM 19766T (96.3%), Nesterenkonia populi KCTC 29119T (95.9%), Nesterenkonia alba CCTCC AB 207011T (95.5%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The draft genome of the isolate was sequenced, which comprised 3,739,891 bp with G + C content of 63 mol%, and was annotated to contain 3614 protein-coding genes, 44 tRNA genes and 5 rRNA genes. Chemotaxonomic analysis indicated that the main respiratory quinones were MK-8 and MK-9, the predominant cellular fatty acids were anteiso-C17:0, anteiso-C15:0 and iso-C16:0, the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol andphosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain MD2T is considered to represent a novel species, for which the name Nesterenkonia ebinurensis sp. nov. is proposed. The type strain is MD2T (= KCTC 52999T = MCCC 1K03343T).

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Authors and Affiliations

  1. Zhejiang University College of Life Sciences Hangzhou, Zhejiang, China

    Nusratgul Anwar, Yanghui Ye, Can Chen, Tursunay Mamtimin, Ruijun Wang, Erkin Rahman & Min Wu

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  1. Nusratgul Anwar
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  2. Yanghui Ye
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Anwar, N., Ye, Y., Chen, C. et al. Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica. Curr Microbiol 78, 3804–3810 (2021). https://doi.org/10.1007/s00284-021-02597-4

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  • DOI: https://doi.org/10.1007/s00284-021-02597-4

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