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Aspergillus fumigatus Induces the Release of IL-8 and MCP-1 by Activating Nuclear Transcription Through Dectin-1 and CR3 Receptors in Alveolar Epithelial Cells

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Abstract

Invasive pulmonary aspergillosis induced by the pathogenic fungus Aspergillus fumigatus is one of the common fatal complications in immunocompromised patients. Lung epithelial cells play an important role in host immune defense against A. fumigatus. However, the interaction between lung epithelial cells and A. fumigatus conidia is not fully understood. In this study, we used the swollen conidia of A. fumigatus to stimulate the type II lung epithelial A549 cells. Results showed that swollen conidia could significantly increase RNA transcription and protein expression of interleukin 8 (IL-8) and monocyte chemoattractant protein 1 (MCP-1), but not TNF-α in A549 cells in a time-dependent manner. Moreover, serum opsonization was able to improve the release of inflammatory factors induced by swollen conidia. Blocking of the dectin-1 or CR3 receptors, or both simultaneously, in the A549 cells could decrease the release of IL-8 and MCP-1. Additionally, blocking dectin-1 or CR3 could inhibit the transcription of nuclear factor NF-κB that was activated by swollen conidia. Here we reported for the first time that dectin-1 and CR3 receptors in A549 cells mediate the release of pro-inflammatory factors IL-8 and MCP-1 induced by A. fumigatus.

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Acknowledgements

The authors thank Dr. M. Moore and Dr. K.J. Kwon-Chung for kindly providing A. fumigatus ATCC13073 strain. This work was supported by the Chinese National Scientific Foundation (Grant Nos. 81273229 and 81471565).

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  1. Yanxi Liu and Zhiqian Li have contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China

    Yanxi Liu & Qun Sun

  2. Department of Hospital Infection Control and Research, Institute of Disease Control and Prevention of PLA, Beijing, China

    Yanxi Liu, Zhiqian Li, Changjian Zhang, Li Han & Xuelin Han

  3. Department of Clinical Laboratory of The 907 Hospital of PLA, Fujian, China

    Yanxi Liu & Zhiqian Li

  4. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai, China

    Shuo Wang

  5. Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Qinghai, China

    Shuo Wang

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  1. Yanxi Liu
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Correspondence to Qun Sun or Xuelin Han.

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Liu, Y., Li, Z., Wang, S. et al. Aspergillus fumigatus Induces the Release of IL-8 and MCP-1 by Activating Nuclear Transcription Through Dectin-1 and CR3 Receptors in Alveolar Epithelial Cells. Curr Microbiol 78, 3474–3482 (2021). https://doi.org/10.1007/s00284-021-02534-5

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