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Neobacillus sedimentimangrovi sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment

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Abstract

Two Gram-stain positive, rod-shaped, endospore-forming, aerobic, thermophilic strains, designated FJAT-2464T and FJAT-52740, were isolated from the sediment collected from Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. The 16S rRNA gene sequence similarity between strains FJAT-2464T and FJAT-52740 was 100%. The result suggests that strains FJAT-2464T and FJAT-52740 belong to the same genome species, hence only FJAT-2464T was considered for further analysis. Strain FJAT-2464T showed the highest 16S rRNA gene sequence similarities to the type strains of Neobacillus thermocopriae SgZ-7T (99.9%), Neobacillus cucumis AP-6T (97.6%) and Neobacillus drentensis LMG 21831T (97.5%). Growth was observed at 25–65 °C (optimum 60 °C), pH 7.0–8.0 (optimum 8.0) with NaCl tolerance up to 1.0% (w/v) (optimum without NaCl %). The cell-wall peptidoglycan contained meso-diaminopimelic acid and MK-7 was the only respiratory quinone. The major fatty acids were iso-C15:0 and anteiso-C15:0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified aminophospholipid and unidentified lipids. The average nucleotide identity and digital DNA-DNA hybridization values between strain FJAT-2464T and the most closely related strain N. thermocopriae SgZ-7T was below the threshold value for species delineation. Based on the above results, strain FJAT-2464T represents a novel species of the genus Neobacillus, for which the name Neobacillus sedimentimangrovi sp. nov. is proposed. The type strain is FJAT-2464T (= MCCC 1K04406T = KCTC 43264T).

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Acknowledgement

We thank Prof. Aharon Oren for the nomenclature help.

Funding

This work was financially supported by the Fujian Academy of Agricultural Sciences (DEC201821209, GYPY2019003 and AGP2018-3).

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Author notes

  1. Rong Tang and Qi Zhang contributed equally to the work.

Authors and Affiliations

  1. Fujian Agricultural and Forestry University, Fuzhou, 350002, Fujian, China

    Rong Tang, Qi Zhang & Shun-Gui Zhou

  2. Agricultural Bio-Resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China

    Guo-Hong Liu, Jian-Mei Che, Min Lei & Bo Liu

  3. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 10275, People’s Republic of China

    Manik Prabhu Narsing Rao & Wen-Jun Li

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Contributions

RT and QZ designed and performed the experiments. MPNR and G-HL performed the genome analysis and revised the manuscript. J-MC and ML re-checked the manuscript. G-MH helped during sample collection. BL, W-JL and S-GZ supervised the study. All authors read and approved the manuscript.

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Correspondence to Wen-Jun Li or Shun-Gui Zhou.

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The authors declare that they have no competing interests.

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We have obtained the appropriate permission from the responsible authorities of Zhangjiang Estuary Mangrove National Nature Reserve, Fujian Province, China, for collecting the samples.

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Tang, R., Zhang, Q., Narsing Rao, M.P. et al. Neobacillus sedimentimangrovi sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment. Curr Microbiol 78, 1039–1044 (2021). https://doi.org/10.1007/s00284-021-02360-9

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