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Flagellimonas hymeniacidonis sp. nov., Isolated from the Sponge Hymeniacidon sinapium

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Abstract

A Gram-stain-negative, rod-shaped, non-motile, aerobic, yellow-pigmented, marine bacterium (designated strain 176CP5-101 T) was isolated from a sponge Hymeniacidon sinapium from Cheongpo beach, Taean in Republic of Korea. The strain was able to grow at pH 6.8–8.5 (optimum at pH 7.5), in 1–7% (w/v) NaCl (optimum at 3%, w/v) and at 10–30 °C (optimum at 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 176CP5-101 T belonged to the family Flavobacteriaceae and was most closely related to Flagellimonas aquimarina KCTC 52351 T (97.1%), F. algicola KACC 19790 T (97.0%), F. pacifica KCTC 52762 T (96.9%), F. eckloniae KCCM 42307 T (96.4%), F. flava KCTC 22665 T (96.0%), and F. maritima KCTC 32464 T (95.4%). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain 176CP5-101 T and F. aquimarina KCTC 52351 T were, respectively, 79.9% and 22.1%, indicating that 176CP5-101 T is a novel species of the genus Flagellimonas. The DNA G + C content of the genomic DNA was 37.8 mol%. Menaquinone with six isoprene units (MK-6) was the major respiratory quinone, and the major polar lipids were phosphatidylethanolamine (PE), unidentified aminophospholipid, and unidentified lipids. The fatty acids are mainly (> 5%) defined iso-C15:0, iso-C15:0 3-OH, iso-C15:1 G, iso-C17:0 3-OH, and C16:1 ω7c and/or C16:1 ω6c (summed feature 3). Phenotypic, phylogenetic, genomic, and chemotaxonomic characteristics showed that strain 176CP5-101 T represents a novel species of the genus Flagellimonas, for which the name Flagellimonas hymeniacidonis sp. nov. is proposed. The type strain is 176CP5-101 T (= KACC 19892 T = LMG 31316 T).

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Acknowledgements

"This research was supported by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170431) and 2019 Hannam University Research Fund.

Funding

The NCBI GenBank accession numbers for the 16S rRNA gene sequence and genome sequence of strain 176CP5-101 T are MK040415 and GCA 008040165.

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  1. Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-Dong, Yuseong-gu, Daejeon, 34430, Republic of Korea

    Tae-Gi Shin & Jin-Sook Park

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  1. Tae-Gi Shin
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Shin, TG., Park, JS. Flagellimonas hymeniacidonis sp. nov., Isolated from the Sponge Hymeniacidon sinapium. Curr Microbiol 78, 1061–1067 (2021). https://doi.org/10.1007/s00284-020-02328-1

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