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Pseudomonas pratensis sp. nov., Isolated from Grassland Soil from Inner Mongolia, China

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Abstract

A novel bacterial strain, designated MHJ-10JT, was isolated from a soil sample obtained from a grassland in Inner Mongolia, China. MHJ-10JT strain could grow at 4–37 °C (optimum: 30 °C) and pH 4–9 (optimum: pH 6), as well as in the presence of 0–6% NaCl (optimum: 1%). Cells of strain MHJ-10JT are Gram-negative, rod-shaped, and motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MHJ-10JT was most closely related to Pseudomonas lutea OK2T (98.5% 16S rRNA gene sequence similarity). The values of the average nucleotide identities (ANI) and digital DNA–DNA hybridization (dDDH) between strain MHJ-10JT and its related species were all below 80.5% and 24.4%, respectively, which are significantly lower than the thresholds of 95% for ANI and 70% for DDH for species delineation. The genomic G + C content of the MHJ-10JT strain is 64.8 mol%. Based on the phenotypic, genotypic, chemotaxonomic, and phylogenetic analyses, strain MHJ-10JT can be assigned to the genus Pseudomonas. In this study, we propose that strain MHJ-10JT be classified as a novel species belonging to the genus Pseudomonas with the species name Pseudomonas pratensis sp. nov. The type strain of the proposed novel species is MHJ-10JT (= KCTC 82206T = CGMCC 17322T).

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Acknowledgements

This work was support by the Science and Technology Major Project on Lakes of Inner Mongolia grant (ZDZX2018054), National Natural Science Foundation of China grants (41563009, 41963008), and the Open Project Program of Ministry of Education Key Laboratory of Ecology and Resources Use of the Mongolian Plateau (10000-196106/001/001). We thank Professor Aharon Oren from The Hebrew University of Jerusalem for the help for nomenclature of bacteria.

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China

    Meng Zhang, Lixin Shen, Jiajia Shi, Linxia Chen, Ying Luo, Jiquan Sun, Deliang Fan & Zhihua Bao

  2. Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, 980-8577, Japan

    Shintaro Hara

  3. Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource Reuse, Inner Mongolia University, Hohhot, 010021, China

    Zhihua Bao

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  1. Meng Zhang
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Zhang, M., Shen, L., Shi, J. et al. Pseudomonas pratensis sp. nov., Isolated from Grassland Soil from Inner Mongolia, China. Curr Microbiol 78, 789–795 (2021). https://doi.org/10.1007/s00284-020-02296-6

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