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Isolation and Characterization of Serratiopeptidase Producing Bacteria from Mulberry Phyllosphere

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Abstract

Serratiopeptidase (EC 3.4.24.40), a proteolytic enzyme, is one of the most promising enzymes being used in biopharmaceutical industry. Mulberry phyllosphere, being an unexplored niche for exploration of protease production, was chosen for the present study. Protease producing bacteria were isolated from the tissues of mulberry plant as well as its rhizospheric soil. Two protease producing bacteria belonging to Serratia genus were found to be potential serratiopeptidase producers. Among them, the endophyte, i.e., Serratia marcescens MES-4 presented 95 Units/mL activity, while the soil isolate i.e., Serratia marcescens MRS-11 presented 156 Units/mL activity.

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Acknowledgements

Authors are thankful to the Director CSIR-IIIM for his interest in this work. Financial support from CSIR Aroma Mission (Grant No. HCP-0007) is gratefully acknowledged. DC and RSM are thankful to UGC and ICMR, New Delhi, for their respective fellowships. The manuscript bears institutional communication no. CSIR-IIIM/IPR00184.

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Authors and Affiliations

  1. Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India

    Diksha Koul, Devtulya Chander, Ravi S. Manhas & Asha Chaubey

  2. Academy of Scientific and Innovative Research, CSIR-Human Resource Development Centre, Campus Ghaziabad, 201002, India

    Devtulya Chander, Ravi S. Manhas & Asha Chaubey

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  1. Diksha Koul
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  2. Devtulya Chander
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  4. Asha Chaubey
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Correspondence to Asha Chaubey.

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Koul, D., Chander, D., Manhas, R.S. et al. Isolation and Characterization of Serratiopeptidase Producing Bacteria from Mulberry Phyllosphere. Curr Microbiol 78, 351–357 (2021). https://doi.org/10.1007/s00284-020-02280-0

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  • DOI: https://doi.org/10.1007/s00284-020-02280-0

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