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Characterization and Genome Analysis of a Novel Mu-like Phage VW-6B Isolated from the Napahai Plateau Wetland of China

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Abstract

Although bacteriophages are more numerous and have smaller genomes than their bacterial hosts, relatively few have their genomes sequenced. Here, we isolated the Pseudomonas fluorescens bacteriophage from Napahai plateau wetland and performed de novo genome sequencing. Based on the previous biological characteristics and bioinformatics analysis, it was determined that VW-6B was a linear double-stranded DNA (dsDNA) phage with 35,306 bp, with 56.76% G+C content and 197 bp tandem repeats. The VW-6B genome contained 46 open-reading frames (ORFs), and no tRNA genes were found. Based on phage genome structure, sequence comparison, and collinear analysis, VW-6B should be classified into the family Siphoviridae and be considered as a member of a new species in the Mu-like phage. The newly isolated bacteriophage can specifically infect P. fluorescens, which further enriches the diversity of known bacteriophages and provides a basis for the subsequent research and application of bacteriophages.

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Data Availability

The datasets generated or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31860147 and 31700324). XJ, YW, QZ, and KQ designed research; ZC and ZX performed research; ZC, ZX, and XJ analyzed data and wrote the paper.

Funding

The National Natural Science Foundation of China (31860147 and 31700324).

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China

    Zihong Cui, Zhiwei Xu, Yunlin Wei, Qi Zhang, Kunhao Qin & Xiuling Ji

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Correspondence to Xiuling Ji.

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Cui, Z., Xu, Z., Wei, Y. et al. Characterization and Genome Analysis of a Novel Mu-like Phage VW-6B Isolated from the Napahai Plateau Wetland of China. Curr Microbiol 78, 150–158 (2021). https://doi.org/10.1007/s00284-020-02277-9

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