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Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii

Current Microbiology volume 77, pages 4000–4015 (2020)Cite this article

Abstract

The function of catalases A and T from the budding yeast Saccharomyces cerevisiae (ScCta1 and ScCtt1) is to decompose hydrogen peroxide (H2O2) to mitigate oxidative stress. Catalase orthologs are widely found in yeast, suggesting that scavenging H2O2 is crucial to avoid the oxidative damage caused by reactive oxygen species (ROS). However, the function of catalase orthologs has not yet been experimentally characterized in vivo. Here, we heterologously expressed Debaryomyces hansenii DhCTA1 and DhCTT1 genes, encoding ScCta1 and ScCtt1 orthologs, respectively, in a S. cerevisiae acatalasemic strain (cta1Δ ctt1Δ). We performed a physiological analysis evaluating growth, catalase activity, and H2O2 tolerance of the strains grown with glucose or ethanol as carbon source, as well as under NaCl stress. We found that both genes complement the catalase function in S. cerevisiae. Particularly, the strain harboring DhCTT1 showed improved growth when ethanol was used as carbon source both in the absence or presence of salt stress. This phenotype is attributed to the high catalase activity of DhCtt1 detected at the exponential growth phase, which prevents intracellular ROS accumulation and confers oxidative stress resistance.

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source and galactose was used as an inducer. The catalase activity (a) was measured as previously described above in Table 4. The spot test analysis after H2O2 exposure (40, 80 and 100 mM) was performed in the S. cerevisiae wild-type plus pYES2 (b) and the acatalasemic plus pYES2::DhCTT1 (c) as previously was described in Fig. 4. The catalase activity (a) and the left panel (b–c) are representative of three independent experiments

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Acknowledgements

We thank Juan Pablo Pardo and James Karkashian for sharing their valuable knowledge and criticizing the writing. Antonio Peña for his generosity and support. Francisco Padilla-Garfias for his great technical assistance and for sharing the protocol to measure ROS. Mónica Ramírez-Hernández, Melissa Páez-Iribas, Tamara Pradel-Bernal and Angeles Cancino-Rodezno for helpful technical assistance. This project was supported by Dirección General de Asuntos del Personal Académico (DGAPA) Grant/Award Number: IN225320 and IA208820 (partially).

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  1. Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Cd. Universitaria, 04510, Coyoacán, Ciudad de México, México

    James González, Román Castillo, Miguel Angel García-Campos, Diego Noriega-Samaniego, Viviana Escobar-Sánchez, Luisa Alba-Lois & Claudia Segal-Kischinevzky

  2. Departamento de Bioquímica, Facultad de Medicina, Facultad de Medicina, Universidad Nacional Autónoma de México. Avenida Universidad 3000, Cd. Universitaria, 04510, Coyoacán, Ciudad de México, México

    Lucero Romero-Aguilar

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LA-L, and CS-K conceived and designed the experiments; JG, RC, MAG-C, DN-S, and VE-S conducted experiments; JG, RC, LR-A, VE-S and CS-K analyzed the data; JG, RC, VE-S and CS-K wrote the manuscript.

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Correspondence to Claudia Segal-Kischinevzky.

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González, J., Castillo, R., García-Campos, M.A. et al. Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii. Curr Microbiol 77, 4000–4015 (2020). https://doi.org/10.1007/s00284-020-02237-3

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Keywords

  • Debaryomyces hansenii
  • Catalases
  • Heterologous yeast expression
  • Oxidative stress
  • Salt stress