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Achromobacter aestuarii sp. nov., Isolated from an Estuary

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Abstract

A novel strain KS-M25T was isolated from estuary water in South Korea. Strain KS-M25T was Gram-staining-negative, strictly aerobic, motile rods-shaped bacterium and showed oxidase- and catalase-positive reactions. Growth of strain KS-M25T was observed at 10–25 °C (optimum, 20 °C), at pH 5.5–9.0 (optimum, pH 7.5), and with 0–6.0% (w/v) NaCl (optimum, 1%). Ubiquinone-8 was identified as the sole isoprenoid quinone and the major fatty acids were C16:0, cyclo–C17:0 and sum in feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c). The G+C content values based on genome sequences was 62.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KS-M25T formed a clear cluster within the genus Achromobacter. Strain KS-M25T was most closely related to Achromobacter anxifer LMG 26857T, Achromobacter dolens LMG 26840T, and Achromobacter xylosoxidans NBRC 15126T with 97.8%, 97.8%, and 97.7% 16S rRNA gene sequence similarities, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic analysis, strain KS-M25T represents a novel species of the genes Achromobacter, for which the name Achromobacter aestuarii sp. nov. is proposed. The type strain is KS-M25T (= KACC 21219T = JCM 33329T).

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References

  1. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntton FM (1923) Bergey’s manual of determinative bacteriology. The Williams & Wilkins Co., Baltimore, USA, Baltimore

    Google Scholar 

  2. Skerman VBD, McGowan V, Sneath PHA (1980) Approved lists of bacterial names. Int J Syst Evol Microbiol 30:225–420

    Article  Google Scholar 

  3. Yabuuchi E, Yano I (1981) Achromobacter gen. nov. and Achromobacter xylosoxidans (ex Yabuuchi and Ohyama 1971) nom. rev. Int J Syst Evol Microbiol 31:477–478

    Google Scholar 

  4. Yabuuchi E, Kawamura Y, Kosako Y, Ezaki T (1998) Emendation of genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kiredjian et al.) comb. nov., and Achromobacter xylosoxidans subsp. denitrificans (Ru¨ger and Tan) comb. nov. Microbiol Immunol 42:429–438

    Article  CAS  PubMed  Google Scholar 

  5. Packer L, Vishniac W (1955) Chemosynthetic fixation of carbon dioxide and characteristics of hydrogenase in resting cell suspensions of Hydrogenomonas ruhlandii nov. spec. J Bacteriol 70:216–223

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Kiredjian M, Holmes B, Kersters K, Guilvout I, Deley J (1986) Alcaligenes piechaudii, a new species from human clinical specimens and the environment. Int J Syst Evol Microbiol 36:282–287

    CAS  Google Scholar 

  7. Rüger HJ, Tan TL (1983) Separation of Alcaligenes denitrificans sp. nov., nom. rev. from Alcaligenes faecalis on the basis of DNA base composition, DNA homology, and nitrate reduction. Int J Syst Evol Microbiol 33:85–89

    Google Scholar 

  8. Kim JM, Le NT, Chung BS, Park JH, Bae JW, Madsen EL, Jeon CO (2008) Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 74:7313–7320

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacterial 173:697–703

    Article  CAS  Google Scholar 

  11. Nawrocki EP, Eddy SR (2007) Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 3:e56

    Article  PubMed  PubMed Central  Google Scholar 

  12. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, He G, Chen Y, Pan Q, Lu Y, Tang J, Wu G, Zhang H, Shi Y, Liu Y, Yu C, Wang B, Lu Y, Han C, Cheung DW, Yiu S, Peng S, Xiaoqian Z, Liu G, Liao X, Li Y, Yang H, Wang J, Lam T, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1:18

    Article  PubMed  PubMed Central  Google Scholar 

  14. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26:24690–32461

    Article  Google Scholar 

  15. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J (2016) JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 32:929–931

    Article  CAS  PubMed  Google Scholar 

  16. Meier-Kolthof JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60

    Article  Google Scholar 

  17. Gomori G (1955) Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146

    Article  CAS  Google Scholar 

  18. Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  19. Lányi B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67

    Google Scholar 

  20. Minnikin DE, O’donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  21. Komagata K, Suzuki KI (1988) 4 Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–208

    Article  Google Scholar 

  22. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark

    Google Scholar 

  23. Bossemeyer D, Borchard A, Dosch DC, Helmer GC, Epstein W, Booth IR, Bakker EP (1989) K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane. J Biol Chem 264:16403–16410

    Article  CAS  PubMed  Google Scholar 

  24. Dosch DC, Helmer GL, Sutton SH, Salvacion FF, Epstein W (1991) Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium. J Bacteriol 173:687–696

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Matsuda N, Kobayashi H, Katoh H, Ogawa T, Futatsugi L, Nakamura T, Bakker EP, Uozumi N (2004) Na+ dependent K+ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC 6803 and its role in the early phases of cell adaptation to hyperosmotic shock. J Biol Chem 279:54952–54962

    Article  CAS  PubMed  Google Scholar 

  26. Ali MK, Li XF, Tang Q, Liu XY, Chen F, Xiao JF, Ali M, Chou SH, He J (2017) Regulation of inducible potassium transporter KdpFABC by the KdpD/KdpE two-component system in Mycobacterium smegmatis. Front Microbiol 8:570

    Article  PubMed  PubMed Central  Google Scholar 

  27. Ness LS, Booth IR (1999) Different foci for the regulation of the activity of the KefB and KefC glutathione-gated K+ efflux systems. J Biol Chem 274:9524–9530

    Article  CAS  PubMed  Google Scholar 

  28. Kung C, Martinac B, Sukharev S (2010) Mechanosensitive channels in microbes. Annu Rev Microbiol 64:313–329

    Article  CAS  PubMed  Google Scholar 

  29. Vimont S, Berche P (2000) NhaA, an Na+/H+ antiporter involved in environmental survival of Vibrio cholerae. J Bacteriol 182:2937–2944

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Kosono S, Haga K, Tomizawa R, Kajiyama Y, Hatano K, Takeda S, Wakai Y, Hino M, Kudo T (2005) Characterization of a multigene-encoded sodium/ hydrogen antiporter (Sha) from Pseudomonas aeruginosa: its involvement in pathogenesis. J Bacteriol 187:5242–5248

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Coenye T, Vancanneyt M, Falsen E, Swings J, Vandamme P (2003) Achromobacter insolitus sp. nov. and Achromobacter spanius sp. nov., from human clinical samples. Int J Syst Evol Microbiol 53:1819–1824

    Article  CAS  PubMed  Google Scholar 

  33. Vandamme P, Moore ER, Cnockaert M, Peeters C, Svensson-Stadler L, Houf K, Spilker T, LiPuma JJ (2013) Classification of Achromobacter genogroups 2, 5, 7 and 14 as Achromobacter insuavis sp. nov., Achromobacter aegrifaciens sp. nov., Achromobacter anxifer sp. nov. and Achromobacter dolens sp. nov., respectively. Syst Appl Microbiol 36:474–482

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2017R1C1B5077091) and the National Institute of Fisheries Science (NIFS, R2020051).

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Authors and Affiliations

  1. Department of Biology, Kunsan National University, 558 Daehak-ro, Gunsan-si, 54150, Republic of Korea

    Si Chul Kim & Hyo Jung Lee

  2. Tidal Flat Research Center-National Institute of Fisheries Science, Gunsan, Republic of Korea

    Sang Ok Chung

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  1. Si Chul Kim
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  2. Sang Ok Chung
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  3. Hyo Jung Lee
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Contributions

SCK isolated the type strain from an estuary and performed the experiments. SOC analyzed the genome. HJL supervised all works and wrote the manuscript. The manuscript has been reviewed and edited by all authors.

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Correspondence to Hyo Jung Lee.

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Kim, S.C., Chung, S.O. & Lee, H.J. Achromobacter aestuarii sp. nov., Isolated from an Estuary. Curr Microbiol 78, 411–416 (2021). https://doi.org/10.1007/s00284-020-02231-9

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