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Decolorization of Textile Effluent by Trametes hirsuta Bm-2 and lac-T as Possible Main Laccase-Contributing Gene

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Abstract

The decolorization of dye and textile effluent by Trametes hirsuta was studied in both induced and non-induced media. A removal of 70–100% of the color was achieved through adsorption and the action of laccases. Laccase activity was increased significantly with the addition of grapefruit peel (4000 U/mL) and effluent with grapefruit peel (16,000 U/mL) in comparison with the basal medium (50 U/mL). Analysis of the expression of laccase isoenzymes lac-B and lac-T revealed clear differences in the expression of these genes. The low levels of expression of lac-B in all media suggest a basal or constitutive gene expression, whereas lac-T was over-expressed in the media with effluent, and showed an up/down regulation depending on culture conditions and time. The results obtained suggest that the lac-T gene of T. hirsuta is involved in the decolorization of dyes.

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Acknowledgements

The names of funding organizations were Consejo Nacional de Ciencia y Tecnología (CONACYT)-FOMIX Award Number 108415 and Tecnológico Nacional de México Award Number 7409.19-P.

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Authors and Affiliations

  1. Renewable Energy Unit, Centro de Investigacion Cientifica de Yucatán, Carretera Sierra Papacal-Chuburna Puerto Km 5, 97302, Mérida, Yucatán, Mexico

    Raul Tapia-Tussell & Liliana Alzate-Gaviria

  2. Department of Chemical and Biochemical Engineering, Tecnologico Nacional de Mexico/IT de Merida, Av. Tecnologico Km 4.5 S/N, 97118, Mérida, Yucatán, Mexico

    Alejandrina Pereira‑Patrón, Gabriel Lizama-Uc & Sara Solis‑Pereira

  3. GeMBio Laboratory, Centro de Investigación Científica de Yucatan, Calle 43 No. 130 x 32 y 34. Col. Chuburna de Hidalgo, 97205, Mérida, Yucatán, Mexico

    Daisy Pérez-Brito

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  1. Raul Tapia-Tussell
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  2. Alejandrina Pereira‑Patrón
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All the authors contributed to this work. T-T and S-P conceived, designed and wrote the paper; P-P performed the experiments and analyzed the data; A-G, P-B, participated in the data analysis and writing of the paper; L-UC in the review and editing.

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Correspondence to Sara Solis‑Pereira.

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Tapia-Tussell, R., Pereira‑Patrón, A., Alzate-Gaviria, L. et al. Decolorization of Textile Effluent by Trametes hirsuta Bm-2 and lac-T as Possible Main Laccase-Contributing Gene. Curr Microbiol 77, 3953–3961 (2020). https://doi.org/10.1007/s00284-020-02188-9

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