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Efficiency of Candida tropicalis for Potential Degradation of Metalaxyl in the Aqueous Media

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Abstract

Biodegradation of pesticides by bacteria, fungi, algae, and other organisms is an eco-friendly, efficient, and economical method of detoxification. This study was conducted to evaluate the efficiency of Candida tropicalis for potential degradation of metalaxyl in the aqueous media. The yeast fungus from soil with a historical application of metalaxyl has been isolated. The identification of isolated fungus was carried out using 28S rRNA. The effect of pH, temperature, and metalaxyl concentration on the growth of C. tropicalis and its ability for metalaxyl degradation was also investigated. The isolated fungus was identified as C. tropicalis based on the sequencing of D1D2 region of the 28S rRNA and BLAST search of the nucleotide sequences. The growth of C. tropicalis in the presence of metalaxyl was significantly higher than in the absence of it. A temperature of 30 °C, a pH value of 7, and metalaxyl concentration of 5 mg/L were the optimal conditions for C. tropicalis to grow and to obtain the highest degradation rate of metalaxyl. At the optimum conditions, the degradation rate and the half-life value of metalaxyl were 0.065 d-1 and 10.7 days, respectively. Under the optimum conditions, a 98% degradation of metalaxyl initial concentration obtained within 36 days. This report is novel in showing the biodegradation of metalaxyl in water by the yeast C. tropicalis, which can be a promising method in this regard.

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Acknowledgements

The authors highly appreciated to Dr. Mohamed Abdelrazek Saleh, Associate Professor of Pesticides Chemistry and Toxicology, Faculty of Agriculture, Kafrelshiekh University for providing his laboratory facilities for microbial growth and degradation measurements.

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Authors and Affiliations

  1. Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Aly Soliman Hamed Derbalah & Mostafa Saad Allah

  2. Genetics Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Antar El-Banna

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  1. Aly Soliman Hamed Derbalah
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  2. Antar El-Banna
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  3. Mostafa Saad Allah
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Contributions

Prof. Antar El-Banna involved in the isolation and identification of the microbe. Prof. Aly Derbalah and Dr. Mostafa Saad Allah involved in the growth kinetics of the identified microbe and biodegradation kinetics of metalaxyl.

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Correspondence to Aly Soliman Hamed Derbalah.

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284_2020_2121_MOESM1_ESM.docx

Fig. 1S The D1–D2 region of the yeast isolate amplified with NL1 and NL4 primers,1, PCR product ~680 bp.; M, Sizer-1000 DNA marker; N, negative control (DOCX 303 kb)

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Derbalah, A.S.H., El-Banna, A. & Saad Allah, M. Efficiency of Candida tropicalis for Potential Degradation of Metalaxyl in the Aqueous Media. Curr Microbiol 77, 2991–2999 (2020). https://doi.org/10.1007/s00284-020-02121-0

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