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Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 Reveals the Presence of Polysaccharide-Degrading Ability Based on Habitat

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Abstract

The genus Hymenobacter is classified in the family Hymenobacteraceae under the phylum Bacteroidetes. They have been isolated from diverse environments, such as air, soil, and lichen, along with extreme polar environments, including the Arctic and Antarctic regions. The polar regions have attracted intense research interest for the discovery of novel microorganisms and their functions. Analysis of the polysaccharide utilization-related carbohydrate-active enzyme among the two lichen-associated polar organisms Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 was performed, along with its comparison with the complete genome of the same genus available in the NCBI database. The study was conducted relying on the AZCL screening data for the two polar lichen-associated species. While comparing with eight other complete genomes, differences in polysaccharide preferences based on the isolation environment and biosample source were discovered. All the species showed almost similar percentage of cellulose synthesis and degradation genes. However, the polar lichen-associated microorganism was found to have a high percentage of hemicellulose degradation genes, and less starch and laminarin degradation. The Hymenobacter species with higher number of hemicellulose degradation genes was found to have a lower number of starch and laminarin degradation genes and vice versa, highlighting the differences in polysaccharide utilization among the species.

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Acknowledgement

This work was supported by the Korea Polar Research Institute (KOPRI Grant No. PM20030).

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Authors and Affiliations

  1. Department of Life Science and Biochemical Engineering, Graduate School, SunMoon University, Asan, 31460, Korea

    Nisha Ghimire, So-Ra Han, Byeollee Kim & Tae-Jin Oh

  2. Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Korea

    Hyun Park

  3. Unit of Research for Practical Application, Korea Polar Research Institute, Incheon, 21990, Korea

    Jun Hyuck Lee

  4. Department of Polar Sciences, University of Science and Technology, Incheon, 21990, Korea

    Jun Hyuck Lee

  5. Genome-Based BioIT Convergence Institute, Asan, 31460, Korea

    Tae-Jin Oh

  6. Department of Pharmaceutical Engineering and Biotechnology, SunMoon University, Asan, 31460, Korea

    Tae-Jin Oh

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  1. Nisha Ghimire
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Correspondence to Jun Hyuck Lee or Tae-Jin Oh.

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Ghimire, N., Han, SR., Kim, B. et al. Comparative Genomic Study of Polar Lichen-Associated Hymenobacter sp. PAMC 26554 and Hymenobacter sp. PAMC 26628 Reveals the Presence of Polysaccharide-Degrading Ability Based on Habitat. Curr Microbiol 77, 2940–2952 (2020). https://doi.org/10.1007/s00284-020-02120-1

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