Skip to main content
SpringerLink
Log in

Laboratory Evaluation of Beauveria bassiana ARP14 Against Grapholita molesta (Lepidoptera: Tortricidae)

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Entomopathogenic fungi often have wide host range and can be important biological control agents against the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), a significant pest of stone and pome fruits. The virulence of six entomopathogenic fungi, including three strains of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) (ARP14, GHA, A) and one strain each of Metarhizium robertsii Bischoff, Rehner, & Humber (Hypocreales: Clavicipitaceae), Metarhizium brunneum (Petch) (Hypocreales: Clavicipitaceae), and Isaria farinosa (Holmsk.) (Hypocreales: Cordycipitaceae) was assessed against first instars (< 5 h old) of G. molesta held in glass scintillation vials. Compared to commercialized strain of B. bassiana (GHA), B. bassiana ARP14, which was recovered from a cadaver of Riptortus pedestris (F.) (Hemiptera: Alydidae), killed first instar larvae 2.6 times faster at 12 h after exposure to 1 × 108 conidia/ml concentration at 95.9% RH and 25.4 °C. However, the mycosis rate after 14 days was similar to that of B. bassiana GHA in all treatments. Beauveria bassiana ARP14 also killed adult moths 2.7 (males) and 2.2 (females) times faster than did B. bassiana GHA, measured 168 h (7 days) after exposure. However, the mycosis rate after 14 days was similar from both fungi. In assay using three conidial concentrations (1 × 107, 1 × 108, and 1 × 109 conidia/ml) and three RHs levels (55, 75, and 95%). Mortality became higher as concentration and RH increase, but mycosis rate at 14 days wasn’t significantly different among treatments. These results suggest that B. bassiana ARP14 can be a potential biological control agent against G. molesta.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Najar-Rodriguez A, Bellutti N, Dorn S (2013) Larval performance of the oriental fruit moth across fruits from primary and secondary hosts. Physiol Entomol 38:63–70

    Google Scholar 

  2. Rothschild GHL, Vickers RA (1991) Biology, ecology and control of the oriental fruit moth. In: van der Geest LPS, Evenhuis HH (eds) Tortricid pests: their biology. Natural Enemies and Control. Elsevier Publishers, New York, pp 389–412

    Google Scholar 

  3. Mazzi D, Dorn S (2012) Movement of insect pests in agricultural landscapes. Ann Appl Biol 160:97–113

    Google Scholar 

  4. Rice RE, Doyle J, Jones RA (1972) Pear as a host of the oriental fruit moth in California. J Econ Entomol 65:1212–1213

    Google Scholar 

  5. Zhao ZR, Wang YG, Yan GY (1989) A preliminary report on the oriental fruit moth in north Jiangsu. Insect Knowl 26:17–19

    Google Scholar 

  6. Kanga LHB, Pree DJ, van Lier JL, Walker GM (2003) Management of insecticide resistance in Oriental fruit moth (Grapholita molesta; Lepidoptera: Tortricidae) populations from Ontario. Pest Manag Sci 59:921–927

    PubMed  CAS  Google Scholar 

  7. Yang CY, Han KS, Boo KS (2001) Occurrence of and damage by the oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae) in pear orchards. Korean J Appl Entomol 40:117–123

    Google Scholar 

  8. Pree DJ, Whitty KJ, Van Driel L, Walker GM (1998) Resistance to insecticides in Oriental fruit moth populations (Grapholita molesta) from the Niagara peninsula of Ontario. Can Entomol 130:245–256

    Google Scholar 

  9. Pree DJ, Whitty KJ, Pogoda MK, Bittner LA (2005) Status of resistance to insecticides in populations of the oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae) in Southern Ontario. J Ent Soc Ont 136:53–70

    Google Scholar 

  10. Hung D-Z, Yang H-J, Li Y-F, Lin C-L, Chang S-Y, Sung F-C et al (2015) The long-term effects of organophosphates poisoning as a risk factor of CVDs: a nationwide population based cohort study. PLoS ONE 10(9):e0137632

    PubMed  PubMed Central  Google Scholar 

  11. Bing LA, Lewis LC (1991) Suppression of Ostrinia nubilalis (Hübner) (Lepidoptera: Pyralidae) by endophytic Beauveria bassiana (Balsamo) Vuillemin. Environ Entomol 20:1207–1211

    Google Scholar 

  12. Vandenberg JD, Shelton AM, Wilsey WT, Ramos M (1998) Assessment of Beauveria bassiana sprays for control of diamondback moth (Lepidoptera: Plutellidae) on crucifers. J Econ Entomol 91:624–630

    Google Scholar 

  13. Nguyen NTH, Borgemeister C, Poehling H, Zimmermann G (2007) Laboratory investigation on the potential of entomopathogenic fungi for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae and pupae. Biocontrol Sci Technol 17:853–864

    Google Scholar 

  14. Duarte RT, Gonçalves KC, Espinosa DJJ, Moreira LF, De Bortoli SA, Humber RA, Polanczyk RA (2016) Potential of entomopathogenic fungi as biological control agents of diamondback moth (Lepidoptera: Plutellidae) and compatibility with chemical insecticides. J Econ Entomol 109:594–601

    PubMed  CAS  Google Scholar 

  15. Wraight SP, Ramos ME, Avery PB, Jaronski ST, Vandenberg JD (2010) Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops. J Invertebr Pathol 103:186–199

    PubMed  CAS  Google Scholar 

  16. Leatherdale D (1970) The arthropod hosts of entomogenous fungi in Britain. Entomophaga 15:419–435

    Google Scholar 

  17. Cossentine JE, Judd GJR, Bissett JD, Lacey LA (2010) Susceptibility of apple clearwing moth larvae, Synanthedon myopaeformis (Lepidoptera: Sesiidae) to Beauveria bassiana and Metarhizium brunneum. Biocontrol Sci Technol 20:703–707

    Google Scholar 

  18. Dangi N, Lim UT (2018) Identification and evaluation of a new entomopathogenic fungal strain against Riptortus pedestris (Hemiptera: Alydidae) and its two parasitoids. PLoS ONE 13:e0195848

    PubMed  PubMed Central  Google Scholar 

  19. Du J, Li G, Xu X, Wu J (2015) Development and fecundity performance of oriental fruit moth (Lepidoptera: Tortricidae) reared on shoots and fruits of peach and pear in different seasons. Environ Entomol 44:1522–1530

    PubMed  CAS  Google Scholar 

  20. Winston PW, Bates DH (1960) Saturated solutions for the control of humidity in biological research. Ecology 41:232–237

    Google Scholar 

  21. Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  22. Institute SAS (2012) SAS user’s guide: statistics. SAS Institute, Cary

    Google Scholar 

  23. Zar JH (2010) Biostatistical analysis, 5th edn. Prentice Hall, Upper Saddle River

    Google Scholar 

  24. Sosa- Gómez DRS, Boucias DG, Nation JL (1997) Attachment of Metarhizium anisoplise to the southern green stink bug Nezara viridula cuticle and fungistatic effect of cuticular lipids and aldehydes. J Invertebr Pathol 69:31–39

    PubMed  Google Scholar 

  25. Sosa- Gómez DRS, Moscardi F (1998) Laboratory and field studies on the infection of stink bugs, Nezara viridula, Piezodorus guildinii, and Euschistus heros with Metarhizium anisoplise and Beauveria bassiana in Brazil. J Invertebr Pathol 71:115–120

    Google Scholar 

  26. Faria M, Wraight SP (2001) Biological control of Bemisia tabaci with fungi. Crop Prot 20:767–778

    Google Scholar 

  27. Feng MG, Chen B, Ying SH (2004) Trails of Beauveria bassiana, Paecilomyces fumosoroseus and imidacloprid for management of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) on greenhouse grown lettuce. Biocontrol Sci Technol 14:513–544

    Google Scholar 

  28. Hatting JL, Wraight SP, Miller RM (2004) Efficacy of Beauveria bassiana (Hyphomycetes) for control of Russian wheat aphid (Homoptera: Aphididae) on resistant wheat under field conditions. Biocontrol Sci Technol 14:459–473

    Google Scholar 

  29. Pu XY, Feng MG, Shi CH (2005) Impact of three application methods on the field efficacy of a Beauveria bassiana-based mycoinsecticide against the false-eye leafhopper, Empoasca vitis (Homoptera: Cicadellidae) in tea canopy. Crop Prot 24:167–175

    Google Scholar 

  30. Sasaki F, Miyamoto T, Yamamoto A, Tamai Y, Yajima T (2012) Relationship between intraspecific variations and host insects of Ophiocordyceps nutans collected in Japan. Mycoscience 53:85–91

    Google Scholar 

  31. Kučera M, Samšiňáková A (1968) Toxins of the entomophagous fungus Beauveria bassiuna. J Invertebr Pathol 12:316–320

    PubMed  Google Scholar 

  32. James RR, Buckner JS, Freeman TP (2003) Cuticular lipids and silver leaf whitefly stage affect conidial germination of Beauveria bassiana and Paecilomyces fumosoroseus. J Invertebr Pathol 84:67–74

    PubMed  CAS  Google Scholar 

  33. Benz G (1987) Environment. In: Fuxa JR, Tanada Y (eds) Epizootiology of insect diseases. Wiley, NY, pp 177–214

    Google Scholar 

  34. Inglis DG, Goettel SM, Butt MT, Strasser H (2001) Use of hyphomycetes fungi for managing insect pests. In: Butt TM, Jackson C, Magan N (eds) Fungi as biocontrol agents. CABI Publishing, Wallingford, pp 23–27

    Google Scholar 

  35. Alves SB, Tamai MA, Rossi LS, Castiglioni E (2005) Beauveria bassiana pathogenicity to the citrus rust mite Phyllocoptruta olievora. Exp Appl Acarol 37:117–122

    PubMed  Google Scholar 

  36. Seiedy M, Saboori A, Allahyari H, Hassanloui RT, Tork M (2010) Laboratory investigation on the virulence of two isolates of the entomopathogenic fungus Beauveria bassiana against the two spotted spider mite Tetranychus urticae (Acari: Tetranychidae). Int J Acarol 36:527–532

    Google Scholar 

  37. Ferron P, Fargues J, Riba G (1991) Fungi as microbial insecticides against pests. In: Arora DK, Ajello L, Mukerji KG (eds) Handbook of applied mycology Humans, Animals and Insects. Marcel Dekker Inc., New York, pp 665–705

    Google Scholar 

  38. Devi KU, Rao CUM (2006) Allee effect in the infection dynamics of the entomopathogenic fungus Beauveria bassiana (Bals) Vuill. on the beetle Mylabris pustulata. Mycopathologia 161:385–394

    PubMed  Google Scholar 

  39. Sandhu SS, Sharma AK, Beniwal V, Goel G, Batra P, Kumar A, Jaglan S, Sharma AK, Malhotra S (2011) Myco-biocontrol of insect pests: factors involved mechanism, and regulation. J Pathol 2012:126819–126829

    Google Scholar 

  40. Hamill RL, Sullivan HR, Gorman M (1969) Determination of pyrrolnitrin and derivatives by gas-liquid chromatography. Appl Microbiol 18:310–312

    PubMed  PubMed Central  CAS  Google Scholar 

  41. Elsworth JF, Grove JF (1977) Cyclodepsipeptides from Beauveria bassiana Bals. Part 1. Beauverolides H and I. J Chem Soc Perkin Trans 13:270–273

    Google Scholar 

Download references

Acknowledgements

We would like to thank Prof. Jae Su Kim at the Department of Agricultural Biology, Chonbuk National University. This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ013362042020)" Rural Development Administration, Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Plant Medicals, Andong National University, Andong, 760-749, Republic of Korea

    Souvic Sarker, Young Ha Woo & Un Taek Lim

Authors
  1. Souvic Sarker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young Ha Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Un Taek Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Un Taek Lim.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sarker, S., Woo, Y.H. & Lim, U.T. Laboratory Evaluation of Beauveria bassiana ARP14 Against Grapholita molesta (Lepidoptera: Tortricidae). Curr Microbiol 77, 2365–2373 (2020). https://doi.org/10.1007/s00284-020-02012-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-020-02012-4

Search

Navigation