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Complete Genome Sequence of Lactobacillus plantarum EM, A Putative Probiotic Strain with the Cholesterol-Lowering Effect and Antimicrobial Activity

Current Microbiology volume 77, pages 1871–1882 (2020)Cite this article

Abstract

Lactobacillus plantarum EM is a probiotic strain with antimicrobial activity, cholesterol-lowering effects, and tolerance to acid and bile. To understand the genetic basis of the probiotic characteristics of this strain, genome sequencing and probiotic-related genetic analysis were performed. The genomic characteristics of L. plantarum EM were confirmed by comparative genomic analysis with 41 probiotic lactic acid bacteria, including 10 L. plantarum strains. L. plantarum EM was shown to contain a circular chromosome of 3,184,808 bp and eight plasmids with various lengths from 5,027 to 76,369 bp. The L. plantarum EM genome had a total of 3560 protein-coding genes, including probiotic-related genes, such as tolerance to acid and bile, temperature stress, and oxidative stress. Comparative genomic analysis showed that L. plantarum EM contained plantaricin and bovicin gene clusters, which are related to antimicrobial activity, and five bile salt hydrolase genes related to serum cholesterol-lowering effects. The genomic analysis confirmed the probiotic properties of L. plantarum EM, and our results indicated that this strain has potential application for use as an industrially important probiotic.

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Data Availability

The data used to support the findings of this study are included within the article.

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Acknowledgements

This work was supported by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (Grant Number 918005-4).

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Affiliations

  1. Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104, Korea

    Eiseul Kim & Hae-Yeong Kim

  2. Department of Food and Nutrition, Chosun University, Gwangju, 61452, Korea

    Hae Choon Chang

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  1. Eiseul Kim
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Contributions

EK performed the experiments, analyzed data and wrote the manuscript. HCC reviewed and edited the manuscript. HYK designed this study, supervised all experiments and reviewed the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hae-Yeong Kim.

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Kim, E., Chang, H.C. & Kim, HY. Complete Genome Sequence of Lactobacillus plantarum EM, A Putative Probiotic Strain with the Cholesterol-Lowering Effect and Antimicrobial Activity. Curr Microbiol 77, 1871–1882 (2020). https://doi.org/10.1007/s00284-020-02000-8

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  • DOI: https://doi.org/10.1007/s00284-020-02000-8