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Antioxidant Activity and Role of Culture Condition in the Optimization of Red Pigment Production by Talaromyces purpureogenus KKP Through Response Surface Methodology

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Abstract

The red pigment production by Talaromyces purpureogenus KKP, a soil isolate, was optimized by response surface methodology (RSM) in the present study. The cultural parameters, such as pH, temperature, dextrose, and peptone concentrations, were optimized for red pigment production using the central composite design (CCD) experimental design. A second-order quadratic model was used to calculate the relationships between the values at different levels of response. The optimum values of the selected variables under coded factors are 6.0, 27 °C, 2.25%, and 1.10% for pH, temperature, dextrose, and peptone, respectively. The selected variables were most effective in the enhancement of red pigment production at optimized culture conditions. In addition to optimization, the antioxidant activity of the pigment isolated in the present study was found to be promising with IC50 value (40 µg/ml). The HRMS data revealed the identification of delphinidin, limonene, 6-hydroxymethyl-7,8-dihydropterin, d-mannose 6-phosphate, and CDP-DG (18:0/18:0). The results of the present investigation will be added to the existing literature of red pigment production and its optimization by T. purpureogenus

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Authors and Affiliations

  1. Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore, 575018, India

    Kishor Kumar Keekan, Sweta Hallur, Prashant Kumar Modi & Rajesh P. Shastry

  2. C206, Marine Biodiscovery Laboratory, School of Chemistry, National University of Ireland, Galway (NUI Galway), University Road, Galway, H91TK33, Ireland

    Kishor Kumar Keekan

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  1. Kishor Kumar Keekan
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Keekan, K.K., Hallur, S., Modi, P.K. et al. Antioxidant Activity and Role of Culture Condition in the Optimization of Red Pigment Production by Talaromyces purpureogenus KKP Through Response Surface Methodology. Curr Microbiol 77, 1780–1789 (2020). https://doi.org/10.1007/s00284-020-01995-4

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