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Variations in the Bacterial Communities in Anastrepha obliqua (Diptera: Tephritidae) According to the Insect Life Stage and Host Plant

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Abstract

Insects have established close relationships with a wide variety of microorganisms, which play a key role in insect ecology and evolution. Fruit flies in the Tephritidae family have economic importance at the global level, including species such as Anastrepha obliqua, which is an important pest in the neotropical region. Although several studies have been performed on the microbiota associated with fruit flies, there are still large gaps in our knowledge about the bacterial communities on the genus Anastrepha. During this study, we used high-throughput sequencing to characterize the bacterial communities of the polyphagous fly A. obliqua, and we evaluated the effect of the life stage (larvae and adults) and host plant (three plant species) on the structure of these communities. Our results show that the bacterial communities in A. obliqua appears to be structured according to the insect life stage and the host plant. The predominant genera belonging to the phylum Proteobacteria were Wolbachia and Enterobacter in both larvae and adults, and they displayed differences in abundance between them, with Wolbachia sp. being more abundant in larvae and Enterobacter sp. being more abundant in adults. Differences in the structures of the bacterial communities were also observed according to the host plant with higher abundance of Enterobacter and Acetobacter bacteria in mango and plum fruits. Based on our results, it can be hypothesized that the bacterial communities on A. obliqua reorganize according to the needs of these insects during their different life stages and could also play an important role in the establishment of this fly species on different host plants. This study represents the first approach to understanding microorganism–insect interactions in fruit flies in Colombia.

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Acknowledgements

We acknowledge the Colciencias program “jovenes investigadores e innovadores por la paz 2017” and the Universidad del Valle for the financial support provided. We are grateful to Elkin Aguirre and Sandra Velasco for their field assistance and contributions to the project. We thank Chrystian Sosa for his help in data analysis. We thank Mauricio Peñuela for a critical reading of the manuscript and his valuable comments on this paper.

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  1. Biology Department (Departamento de Biología), Universidad del Valle, Street 13 No. 100-00, Cali, 760032, Colombia

    Jenny J. Gallo-Franco & Nelson Toro-Perea

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  1. Jenny J. Gallo-Franco
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Gallo-Franco, J.J., Toro-Perea, N. Variations in the Bacterial Communities in Anastrepha obliqua (Diptera: Tephritidae) According to the Insect Life Stage and Host Plant. Curr Microbiol 77, 1283–1291 (2020). https://doi.org/10.1007/s00284-020-01939-y

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