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Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6

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Abstract

A radio-resistant bacterium labeled as strain TMC-6 was isolated from Thal desert, Pakistan and identified through 16S rRNA gene sequencing as Bacillus indicus strain TMC-6 (MN721293). The isolate was found to be resistant to UV radiation dose of 6.780 × 103 J/m2 and showed 50% survivability to mitomycin C (6 μg/ml) and H2O2 (30 mM). The bacterium showed yellowish orange coloration when grown on tryptone yeast glucose (TGY) medium. The cellular metabolite was extracted in methanol and purified through solid phase extraction with C18 column cartridge. The compound was characterized through UV/Visible spectrophotometry, Fourier Transform Infra-Red (FT-IR) spectroscopy and Liquid Chromatography Mass Spectrometry (LC–MS). The LC–MS analysis of the compound revealed a molar mass of 769 [m/z]− that matched the chemical formula C34H42O20 and identified as a glycosylated flavonoid xanthorhamnin. The compound showed significant antioxidant (77.05%) and metal chelation (79.80%) activities. Xanthorhamnin showed promising oxidative damage inhibitory actions in bovine serum albumin (65.32%) and mice liver lipids (71.61%) and prevented DNA strand breaks from oxidative stress. Cytotoxicity in brine shrimp larvae was observed when compared with mitomycin C indicating its effect toward cancerous cells. These findings concluded that xanthorhamnin from radio-resistant Bacillus indicus strain TMC-6 has high antioxidant, radioprotective, and antitumor properties against UV-mediated oxidative damages.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Ayesha Ahmed Chaudhri, Mahnoor Nadeem, Asim ur Rahman, Tayyaba Alam, Fariha Hasan, Malik Badshah, Samiullah Khan & Aamer Ali Shah

  2. Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, 46000, Pakistan

    Wasim Sajjad

  3. Department of Biotechnology, University of Balochistan, Quetta, Pakistan

    Fazal Rehman

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  1. Ayesha Ahmed Chaudhri
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  2. Mahnoor Nadeem
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  3. Asim ur Rahman
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  4. Tayyaba Alam
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  10. Aamer Ali Shah
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Correspondence to Aamer Ali Shah.

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Chaudhri, A.A., Nadeem, M., Rahman, A.u. et al. Antioxidative and Radioprotective Properties of Glycosylated Flavonoid, Xanthorhamnin from Radio-Resistant Bacterium Bacillus indicus Strain TMC-6. Curr Microbiol 77, 1245–1253 (2020). https://doi.org/10.1007/s00284-020-01930-7

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