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First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak

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Abstract

Lactococcosis is a disease encountered in a wide variety of fish species causing mortalities and having great economic impact on farmed fish. In this study, we report for the first time the isolation of a strain of the recently described novel species Lactococcus petauri, from rainbow trout suffering from lactococcosis. The aim of this study was to determine the complete genome sequence of L. petauri strain LG_SAV_20 and to characterize its antimicrobial resistance and virulence. The genome of L. petauri LG_SAV_20 consists of 2,078,949 base pair (bp) with a GC content of 38.05%, 1950 predicted coding sequence (CDS), and 60 RNAs (51 tRNAs, 3 ncRNAs, and 6 rRNAs). Phylogenetic analysis revealed that L. petauri LG_SAV_20 shares most of its genome with L. garvieae strains isolated from rainbow trout. Detection of genes associated with antimicrobial resistance indicated that the isolate possesses the multidrug transporter mdt(A) gene, while using comparative analysis we identified several genes that might be related to bacterial pathogenesis. This genomic information provides new insights into the role of this novel species as an etiological agent of lactococcosis.

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Authors and Affiliations

  1. Hellenic Agricultural Organisation-DEMETER, Veterinary Research Institute of Thessaloniki, Campus of Thermi, 570 01, Thermi, Greece

    Charalampos Kotzamanidis, Konstantina Bitchava, George Vafeas & Antonios Zdragas

  2. School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Andigoni Malousi, Dimitris Chatzidimitriou, Evaggelia Papadimitriou & Fani Chatzopoulou

  3. Department of Microbiology, AHEPA University General Hospital of Thessaloniki, S. Kiriakidi Str. 1, 546 36, Thessaloniki, Greece

    Lemonia Skoura

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  1. Charalampos Kotzamanidis
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Correspondence to Charalampos Kotzamanidis.

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All the animal procedures were performed in compliance with the Greek legislation and the protocol was approved by the Ethical Committee of the Veterinary Research Institute, ELGO-Demeter.

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Kotzamanidis, C., Malousi, A., Bitchava, K. et al. First Report of Isolation and Genome Sequence of L. petauri Strain from a Rainbow Trout Lactococcosis Outbreak. Curr Microbiol 77, 1089–1096 (2020). https://doi.org/10.1007/s00284-020-01905-8

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