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Transcriptome Analysis Reveals the Effects of Chinese Chive (Allium tuberosum R.) Extract on Fusarium oxysporum f. sp. radicis-lycopersici Spore Germination

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Abstract

Fusarium oxysporum f. sp. radicis-lycopersici (Forl) causes Fusarium crown and root rot of tomato, leading to severe yield losses. Chinese chive and the Chinese chive extract reportedly have antifungal effects. In this study, Chinese chive extract treatments inhibited Forl spore germination, with an EC50 of 0.40 g ml−1 in vitro. Furthermore, the mechanism underlying the fungicidal effects of the Chinese chive extract was analyzed by RNA sequencing. A total of 1252 differentially expressed genes (DEGs) were detected, of which 396 were upregulated and 856 were downregulated. The DEGs were related to starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, galactose metabolism, fatty acid metabolism, sphingolipid metabolism, glycerophospholipid metabolism, peroxisomes, ribosome biogenesis in eukaryotes, mismatch repair, and the phosphatidylinositol signaling system, implying these pathways contribute to the fungicidal activity of the Chinese chive extract. The qRT-PCR results verified the accuracy of the RNA sequencing data. Thus, the Chinese chive extract can inhibit Forl spore germination by affecting spore nutrient metabolism.

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Acknowledgements

We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.

Funding

This work was supported by Shandong Province Natural Science Foundation, China [ZR2014CQ034], Shandong Province Agricultural Fine Variety Project, China [2017LZGC013], Shandong Province Modern Agricultural Industry Technology System, Vegetable Industry Innovation Team, China [SDAIT-05-02], and Qingdao Livelihood Science and Technology Plan, China [18-6-1-114-nsh and 19-6-1-60-nsh]. This research work was carried out in Qingdao Key Laboratory of Genetic Improvement and Breeding of Horticultural Plants, China.

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  1. Xiu Zhang and Hui Wang have contributed equally.

Authors and Affiliations

  1. College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China

    Xiu Zhang, Hui Wang, Wenying Zhu, Wenli Li & Fu Wang

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FW was involved in the conception and design of the study, data analysis, draft and critical revision of article, and final approval; XZ and HW were involved in data acquisition, data analysis, draft and critical revision of article, and final approval, and contributed equally; WYZ and WLL were involved in data analysis, draft and critical revision of article, and final approval.

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Correspondence to Fu Wang.

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Zhang, X., Wang, H., Zhu, W. et al. Transcriptome Analysis Reveals the Effects of Chinese Chive (Allium tuberosum R.) Extract on Fusarium oxysporum f. sp. radicis-lycopersici Spore Germination. Curr Microbiol 77, 855–864 (2020). https://doi.org/10.1007/s00284-020-01875-x

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