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Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate

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Abstract

This study investigated the enzymatic saccharification of alkaline-pretreated sugarcane bagasse (PSB) and the bioconversion of simple sugars from hydrolysates to ethanol and other bioproducts by the yeast Galactomyces geotrichum. The effects of percentage of dry substrate (3 and 10% w/v) and time of hydrolysis (24 and 72 h) in the content of released sugars were evaluated. The concentrations of monosaccharides and total reducing sugars (TRS) were calculated by high-performance liquid chromatography (HPLC) and by 3.5-dinitrosalicylic acid (DNS) method, respectively. The highest concentrations of TRS, glucose and xylose (73.96, 31.78 and 10.85 g/L, respectively) were obtained after the saccharification of 10% of PSB with Cellic CTec3 multi-enzyme cocktail (10 FPU/g cellulose) during 72 h (hydrolysate IV). G. geotrichum UFVJM-R150 fermented both glucose and xylose from the hydrolysates. The most efficient ethanol production was obtained after the fermentation of hydrolysate IV (9.99 g/L of ethanol, volumetric productivity—QP of 0.42 g/L.h and yield of ethanol as a function of the substrate—YP/S of 0.27 gethanol/gsugar). Besides ethanol, G. geotrichum was also able to produce other high-value chemicals such as isoamyl alcohol and galacturonic acid. This is the first report of the potential of the yeast G. geotrichum to fermentate sugarcane bagasse hydrolysates with the production of important bioproducts to further use by biorefineries.

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Acknowledgements

The authors are grateful to Federal University of Uberlândia (UFU) and Minas Gerais State Research Funding Agency (FAPEMIG Grant Number APQ-01360–14) for the financial support.

Funding

This study received financial support from the Minas Gerais State Research Funding Agency (FAPEMIG Grant Number APQ-01360–14).

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Authors and Affiliations

  1. Federal University of Uberlândia, Agricultural Sciences Institute (ICIAG-UFU), Uberlândia, Minas Gerais, 38405-320, Brazil

    Kênia Francisca Resende Lamounier, Patrisia de Oliveira Rodrigues & Milla Alves Baffi

  2. Federal University of Uberlândia, Chemical Institute (IQ-UFU), Uberlândia, Minas Gerais, 38400-902, Brazil

    Daniel Pasquini

  3. Department of Basic Sciences (UFVJM), Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, 39803-371, Brazil

    Alexandre Soares dos Santos

Authors
  1. Kênia Francisca Resende Lamounier
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  2. Patrisia de Oliveira Rodrigues
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  3. Daniel Pasquini
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  4. Alexandre Soares dos Santos
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  5. Milla Alves Baffi
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Contributions

K.F.R. Lamounier designed and performed the experiments, analyzed the data and wrote the manuscript. P.O. Rodrigues and A.S. Santos contributed to perform the experiments. D. Pasquini contributed with funding support and analysis of the data. M.A. Baffi supervised the design and development of research, the analysis of the data and the writing of the manuscript. All authors have approved the final manuscript.

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Correspondence to Milla Alves Baffi.

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The authors declared no conflicts of interest with respect to the research, authorship, and/or publication of this article.

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Lamounier, K.F.R., Rodrigues, P.O., Pasquini, D. et al. Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate. Curr Microbiol 77, 738–745 (2020). https://doi.org/10.1007/s00284-019-01866-7

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