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Identification of Differentially Expressed Genes in Trichoderma koningii IABT1252 During Its Interaction with Sclerotium rolfsii

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Abstract

Sclerotium rolfsii, a soil-borne fungal pathogen, infects more than 500 crop species and causes stem rot/collar rot/seed rot/southern blight/wilt in a wide variety of crops which results in significant yield loses. Presently, antagonistic microbes are gaining more importance in managing plant pathogens because they control the pathogen in an environment-friendly manner. Trichoderma is an antagonistic fungi and most popularly used biocontrol agent against phytopathogenic fungi. It is predominantly used to treat soil and seed for the control of Sclerotium rolfsii infestation. In this study, the Trichoderma koningii IABT1252 that performed better in controlling groundnut seed/ seedling rot caused by S. rolfsii in pot experiments were selected to know the molecular basis for the control. Differentially expressed genes in Trichoderma at two different stages of interaction (prior to contact and after contact with S. rolfsii) were identified. In both the stages, some of the differentially expressed genes included ones coding for hydrolytic enzymes, secondary metabolite biosynthesis, transcription factors, signaling proteins, transporter proteins, and proteins involved in mycoparasitic process of Trichoderma.

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Authors and Affiliations

  1. Department of Biotechnology, College of Agriculture, UAS Dharwad, Karnataka, 580005, India

    Chidanand Rabinal

  2. Department of Genetics and Plant Breeding, College of Agriculture, UAS Dharwad, Karnataka, 580005, India

    Sumangala Bhat

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  1. Chidanand Rabinal
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  2. Sumangala Bhat
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Correspondence to Chidanand Rabinal.

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Rabinal, C., Bhat, S. Identification of Differentially Expressed Genes in Trichoderma koningii IABT1252 During Its Interaction with Sclerotium rolfsii. Curr Microbiol 77, 396–404 (2020). https://doi.org/10.1007/s00284-019-01838-x

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