Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan


A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0–8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia and Chryseolinea genera with 91.2–92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of YsT and related strains were less than 50%. Phenotypic analyses suggested that YsT could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318T = LMG 30359T), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).

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  1. 1.

    Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477

    CAS  Article  Google Scholar 

  2. 2.

    Khan AA, Nawaz MS, Robertson L et al (2001) Identification of predominant human and animal anaerobic intestinal bacterial species by terminal restriction fragment patterns (TRFPs): a rapid, PCR-based method. Mol Cell Probes 15:349–355

    CAS  Article  Google Scholar 

  3. 3.

    Kim JJ, Alkawally M, Brady AL et al (2013) Chryseolinea serpens gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from soil. Int J Syst Evol Microbiol 63:654–660

    CAS  Article  Google Scholar 

  4. 4.

    Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    CAS  Article  Google Scholar 

  5. 5.

    Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Method Microbiol 19:161–207

    CAS  Article  Google Scholar 

  6. 6.

    Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    CAS  Article  Google Scholar 

  7. 7.

    Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. Wiley, Chichester, pp 115–147

    Google Scholar 

  8. 8.

    Lechevalier MP, Debievre C, Lechevalier H (1977) Chemotaxonomy of aerobic Actinomycetes—phospholipid composition. Biochem Syst Ecol 5:249–260

    CAS  Article  Google Scholar 

  9. 9.

    Maejima Y, Kushimoto K, Muraguchi Y, Fukuda K, Miura T, Yamazoe A, Kimbara K, Shintani M (2018) Proteobacteria and Bacteroidetes are major phyla of filterable bacteria passing through 0.22 mum pore size membrane filter, in Lake Sanaru, Hamamatsu, Japan. Biosci Biotechnol Biochem 82(7):1260–1263

  10. 10.

    Minnikin DE, Odonnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2(5):233–241

    CAS  Article  Google Scholar 

  11. 11.

    Qin QL, Xie BB, Zhang XY et al (2014) A proposed genus boundary for the prokaryotes based on genomic insights. J Bacteriol 196:2210–2215

    Article  Google Scholar 

  12. 12.

    Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note101. MIDI Inc, Newark, DE

    Google Scholar 

  13. 13.

    Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526

    CAS  Google Scholar 

  14. 14.

    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    CAS  Article  Google Scholar 

  15. 15.

    Turner S, Pryer KM, Miao VP et al (1999) Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. J Eukaryot Microbiol 46:327–338

    CAS  Article  Google Scholar 

  16. 16.

    Wang JJ, Chen Q, Li YZ (2018) Chryseolinea flava sp. nov., a new species of Chryseolinea isolated from soil. Int J Syst Evol Microbiol 68:3518–3522

    CAS  Article  Google Scholar 

  17. 17.

    Weimann A, Mooren K, Frank J, Pope PB, Bremges A, McHardy AC (2016) From genomes to phenotypes: traitar, the microbial trait analyzer. mSystems 1(6): e00101-16.

  18. 18.

    Yoon JH, Kang SJ, Lee SY et al (2011) Ohtaekwangia koreensis gen. nov., sp. nov. and Ohtaekwangia kribbensis sp. nov., isolated from marine sand, deep-branching members of the phylum Bacteroidetes. Int J Syst Evol Microbiol 61:1066–1072

    CAS  Article  Google Scholar 

  19. 19.

    Yoon SH, Ha SM, Kwon S et al (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617

    CAS  Article  Google Scholar 

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This study was supported by the Institute for Fermentation, Osaka (IFO), Japan.

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Correspondence to Masaki Shintani.

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Maejima, Y., Iino, T., Muraguchi, Y. et al. Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan. Curr Microbiol 77, 306–312 (2020).

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