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Isolation and Complete Genome of the Marine Pseudoalteromonas Phage C7 from Coastal Seawater of Yellow Sea, China

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Abstract

A novel Pseudoalteromonas atlantica phage C7 was isolated from the coastal surface water in the Yellow Sea of China using the agar overlay method. Transmission electron microscopy analysis reveals that it belongs to Siphoviridae with a head of 56 nm in diameter and a tail of 102 nm in length. Microbiological characterization suggests that the phage is stable at a range of temperatures (from − 20 to 65 °C) and the optimal pH range is 6–12. One-step growth curve shows a 45-min latent period and a 105-min rise period. Genomic analysis shows that C7 has a linear, double-stranded 42,261-bp DNA molecule with 40.6% GC content and 76 putative open reading frames (ORFs) with one tRNA. The ORFs are classified into six functional groups as follows: hypothetical protein, phage structure, phage packaging, DNA replication and regulation, transcription, and some additional functions. There are many obviously similarities between C7 and a previously published Pseudoalteromonas phage vB_PspS-H40/1 by genomic comparison. This study provides an important data for further research on the interaction between marine bacteriophages and their hosts.

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Acknowledgements

The research is funded by the Marine Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-6, 2018SDKJ0104-4, 2016ASKJ14), National Key Research and Development Program of China (2017YFA0603200, 2018YFC1406704), the National Natural Science Foundation of China (41676178, 31500339 and 41076088), State Oceanic Administration People’s Republic of China (GASI-02-PAC-ST-MSwin, GASI-02-PAC-ST-MSaut), and Fundamental Research Funds for the Central University of Ocean University of China (201812002, 201762017 and 201562018).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Qi Wang, Yundan Liu, Qian Liu, Xinxin Liu, Fan Yang, Min Wang, Hongbing Shao & Yong Jiang

  2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China

    Min Wang & Yong Jiang

  3. Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao, 266003, China

    Min Wang & Yong Jiang

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  1. Qi Wang
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  2. Yundan Liu
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  3. Qian Liu
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  4. Xinxin Liu
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  5. Fan Yang
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  6. Min Wang
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  7. Hongbing Shao
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  8. Yong Jiang
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Correspondence to Yong Jiang.

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Wang, Q., Liu, Y., Liu, Q. et al. Isolation and Complete Genome of the Marine Pseudoalteromonas Phage C7 from Coastal Seawater of Yellow Sea, China. Curr Microbiol 77, 279–285 (2020). https://doi.org/10.1007/s00284-019-01815-4

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  • DOI: https://doi.org/10.1007/s00284-019-01815-4

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