Analysis of the Microbiota in the Fecal Material of Painted Turtles (Chrysemys picta)

Abstract

We used high-throughput sequencing analysis, which targeted the hypervariable V3–V4 region of the bacterial 16S rRNA gene, to investigate the microbiota in fecal material from ten wild painted turtles (Chrysemys picta) captured in southeastern Wisconsin. The most predominant bacterial phylum detected in all samples was the Firmicutes (relative abundance for all samples 96.4% to 68.3%). The next most predominant phylum was Bacteroidetes (relative abundance for all samples 23.9% to 7.8%) in eight samples. Fusobacteria (relative abundance for all samples 22.2% to 0%) was the second most predominant in the other two samples.

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Acknowledgements

This research was done as part of a Provost Honors project under the leadership of Zina Haywood, Executive Vice President/Provost. We thank Karina Rebman and Jarod Lorenz for their assistance with turtle surveys. We also thank Jennifer Cumpston and Donald Zakutansky for their enthusiastic support of this research.

Funding

This project was supported by funding provided by Gateway Technical College and by the Gateway Foundation.

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Correspondence to Richard William McLaughlin.

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Fugate, H.M., Kapfer, J.M. & McLaughlin, R.W. Analysis of the Microbiota in the Fecal Material of Painted Turtles (Chrysemys picta). Curr Microbiol 77, 11–14 (2020). https://doi.org/10.1007/s00284-019-01787-5

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