Analysis of the Microbiota in the Fecal Material of Painted Turtles (Chrysemys picta)

  • Hannah M. Fugate
  • Joshua M. Kapfer
  • Richard William McLaughlinEmail author


We used high-throughput sequencing analysis, which targeted the hypervariable V3–V4 region of the bacterial 16S rRNA gene, to investigate the microbiota in fecal material from ten wild painted turtles (Chrysemys picta) captured in southeastern Wisconsin. The most predominant bacterial phylum detected in all samples was the Firmicutes (relative abundance for all samples 96.4% to 68.3%). The next most predominant phylum was Bacteroidetes (relative abundance for all samples 23.9% to 7.8%) in eight samples. Fusobacteria (relative abundance for all samples 22.2% to 0%) was the second most predominant in the other two samples.



This research was done as part of a Provost Honors project under the leadership of Zina Haywood, Executive Vice President/Provost. We thank Karina Rebman and Jarod Lorenz for their assistance with turtle surveys. We also thank Jennifer Cumpston and Donald Zakutansky for their enthusiastic support of this research.


This project was supported by funding provided by Gateway Technical College and by the Gateway Foundation.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

284_2019_1787_MOESM1_ESM.docx (66 kb)
Supplementary file1 (DOCX 66 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.General Studies, Gateway Technical CollegeKenoshaUSA
  2. 2.Department of Biological SciencesUniversity of Wisconsin-WhitewaterWhitewaterUSA

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