Vibrio chemaguriensis sp. nov., from Sundarbans, Bay of Bengal

Abstract

A new species of Vibrio (annotated as SBOTS_Iso1) was isolated in August 2014 from the Stn1 located in Chemaguri creek of Sundarbans mangrove ecoregion and taxonomically characterized using a polyphasic approach. Phenotypic analysis including biochemical tests and growth across a wide range of salinities indicated the typical estuarine characteristics of this new species. The bacterium was Gram negative, rod-shaped, oxidase and catalase negative and grows in the presence of NaCl. FAME analysis indicated 31.7% of the cellular fatty acids to be made up of 16:1 ω7c/16:1 ω6c. Amplification and sequencing of 16S rRNA and multilocus sequence analysis of four loci (2040 bp; rpoA, topA, mreB, pyrH) and additional sequence data of ftsZ, atpD, ompW and rpoB genes showed this isolate to be a member of Harveyi clade of the genus Vibrio. The closest phylogenetic neighbour was Vibrio alginolyticus ATCC 17749T with 96.8% similarity. Whole-genome sequence data indicates the presence of ~ 5 Mbp genome. GGDC, orthoANIu and AAI indicated 45%, 92% and 0.962 identity respectively with genome of Vibrio alginolyticus ATCC 17749T. The isolate SBOTS_Iso1 has been named Vibrio chemaguriensis sp. nov. on the name of the site from where it was first isolated.

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Abbreviations

MG356329:

Accession number of 16S rRNA sequence

SHOE00000000:

Whole-genome sequence accession number

TA000574:

Digital Protologue number

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Acknowledgements

Anwesha Ghosh is the recipient of IISER Kolkata Integrated Ph.D. Fellowship. This work is partly supported by grants from IISER Kolkata as well as from WWF-India awarded to Punyasloke Bhadury.

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Supplementary material 1 (DOCX 26 kb)

Supplementary material 2 Fig. S1 The top left panel indicated the cell size and shape as observed under 100× magnification using a light microscope. The top right panel shows DAPI stained image from binding of the dye to the nuclei acid of SBOTS_Iso1 as observed under fluorescent microscopy. The lower left panel shows single cells of SBOTS_Iso1 as observed under FESEM. The lower right panel shows TEM image. The scale bar and magnification is indicated in each image (JPEG 3932 kb)

Supplementary material 3 Fig. S2 The OD (600 nm) versus time (h) values obtained during the growth curve experiment performed at different salinities. Growth of the isolate at salinity 11.5, temperature 32 °C and pH 8 indicated the in situ conditions of the study site during sampling (TIFF 2607 kb)

Supplementary material 4 Fig. S3 The OD (600 nm) versus media pH shows growth of the isolate at different media pH levels at salinity 11.5 and incubation at 32 °C (TIFF 11 kb)

Supplementary material 5 Fig. S4 Circular genome of the SBOTS_Iso1 (TIFF 234 kb)

Supplementary material 6 Fig. S5a Alignment between genomes of V. alginolyticus ATCC17749T and SBOTS_Iso1 (TIFF 254 kb)

Supplementary material 7 Fig. S5b Circular genome map indicating ORFs, GC content, GC skew and alignment genome7 (TIFF 1695 kb)

Supplementary material 8 Fig. S6 Genome map showing the position of genomic islands (typically > 8 kb) as seen against V. alginolyticus ATCC17749T reference genome. Genes within these genomic islands might be linked to antibiotic resistance or virulence (TIFF 130 kb)

Supplementary material 9 Fig. S7 Results indicating in silico phenotyping obtained from genome sequence of SBOTS_Iso1 (TIFF 1801 kb)

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Ghosh, A., Bhadury, P. Vibrio chemaguriensis sp. nov., from Sundarbans, Bay of Bengal. Curr Microbiol 76, 1118–1127 (2019). https://doi.org/10.1007/s00284-019-01731-7

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