Pseudomonas ovata sp. nov., Isolated from the Skin of the Tail of Farmed Murray cod (Maccullochella peelii peelii) with a Profound Ulceration

Abstract

A Gram-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagellum, designated as F51T, was isolated from the skin ulcer of farmed Murray cod sampled from Zhejiang Province, China. Strain F51T grew at 4–37 °C (optimal temperature, 28 °C), pH 5.0–8.5 (optimal pH, 7.5) and NaCl concentration of 0–6.0% (w/v) (optimal concentration, 2.0%). Phylogenetic analysis based on average nucleotide identity (76.2–78.4%) and in silico DNA–DNA hybridization (22.3–23.2%) values revealed that strain F51T forms a distinct lineage in the clade of genus Pseudomonas with less than 98.9% 16S rRNA gene sequence similarity to type strains of the genus and represents a novel species related most closely to Pseudomonas floridensis LMG 30013T. Three housekeeping genes (rpoB, rpoD and gyrB) of strain F51T were analysed to further confirm that the isolate is distinctly delineated from related Pseudomonas species. Chemotaxonomic analysis indicated that the sole respiratory quinone of strain F51T is Q-9; its predominant cellular fatty acids are C16:0, summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C10:0 3-OH; and its major polar lipids consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, three unidentified phospholipids and an unidentified aminophosphoglycolipid. This composition is typical of the chemotaxonomic attributes of Pseudomonas. Based on its phenotypic, chemotaxonomic and phylogenetic features, strain F51T is considered to represent a novel species for which the name Pseudomonas ovata sp. nov. is proposed. The type strain is F51T (= KCTC 62133T = MCCC 1K03458T).

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Acknowledgements

This work was financially supported by the Natural Science Foundation of Fujian Province of China (No. 2017J01051), Aquaculture Technology Service Team (No. kjFW19), NDRC Investment Project of Fujian (No. [2016] 482) and the Agricultural Product Quality Safety Innovation Team Project of FAAS (No. STIT2017-1-12).

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Correspondence to Tuyan Luo.

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All the experiments were performed in compliance with the Chinese legislation on the use and care of laboratory animals, and were approved by the Ethical Committee of experiments Animal Care at laboratory animal management office of Zhejiang province (Approval No: SYXK 2013-0191).

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, gyrB, rpoB and rpoD gene sequences of strain F51T are MH305533, MH684587, MH684586 and MH684588, and the DPD number is TA00862. Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession QFFU00000000. The version described in this paper is version QFFU01000000.

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Rao, Q., Liu, Y., Chen, C. et al. Pseudomonas ovata sp. nov., Isolated from the Skin of the Tail of Farmed Murray cod (Maccullochella peelii peelii) with a Profound Ulceration. Curr Microbiol 76, 1168–1174 (2019). https://doi.org/10.1007/s00284-019-01729-1

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