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Prevotella koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion

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Abstract

A novel Gram-negative, obligately anaerobic, non-motile, non-spore forming, and rod-shaped bacterium, designated strain JS262T, was isolated from human subgingival plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. Comparison of 16S ribosomal RNA gene (16S rDNA) sequence revealed that the strain belonged to the genus Prevotella. The percent similarity of 16S rDNA of strain JS262T was closest to those of Prevotella buccae ATCC 33574T (89.1%) and Prevotella shahii JCM 12083T (88.9%). The major fatty acids of strain JS262T were C16:0 (29.2%), iso-C15:0 (19.2%), and anteiso-C15:0 (16.9%). Complete genome of strain JS262T was 2,691,540 bp in length and the G+C content was 43.9 mol%. Average nucleotide identity and genome-to-genome distance values between strain JS262T and P. buccae ATCC 33574T or P. loescheii DSM 19665T were > 70.4% and > 30.1%, respectively. On the basis of these data, a novel Prevotella species is proposed: Prevotella koreensis sp. nov. The type strain of P. koreensis is JS262T (= KCOM 3155T = JCM 33298T).

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Acknowledgements

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science and ICT (Grant No. 2017M3A9B8065844), in part by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (Grant No. 2017R1A2B4004894), and in part the KRIBB Research Initiative Program funded by the Ministry of Science, ICT and Future Planning.

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Author notes

  1. Soon-Nang Park and Yun Kyong Lim have contributed equally to this study.

Authors and Affiliations

  1. Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea

    Soon-Nang Park, Yun Kyong Lim, Eojin Jo & Joong-Ki Kook

  2. Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea

    Jeong Hwan Shin

  3. ABS Research Support Center, KRIBB, Daejeon, Republic of Korea

    Young-Hyo Chang, Yeseul Shin & Jayoung Paek

  4. Department of Periodontics, Institute of Oral Health Science, Ajou University School of Medicine, Suwon, Republic of Korea

    Suk Ji

  5. Vitabio, Inc, Daejeon, Republic of Korea

    Hongik Kim

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  1. Soon-Nang Park
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DPD number: TA00867

GenBank accession number of 16S rRNA gene for strain JS262T: MK452274

GenBank accession number of genome for strain JS262T: RYYU00000000

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Park, SN., Lim, Y.K., Shin, J.H. et al. Prevotella koreensis sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion. Curr Microbiol 76, 1055–1060 (2019). https://doi.org/10.1007/s00284-019-01720-w

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