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The OmpA Gene of Xanthomonas axonopodis pv. glycines is Involved in Pathogenesis of Pustule Disease on Soybean

Current Microbiology volume 76, pages 879–887 (2019)Cite this article

Abstract

The goal of this study was to elucidate the role of the outer membrane protein A (ompA) gene of Xanthomonas axonopodis pv. glycines in bacterial pustule pathogenesis of soybean. An ompA mutant of X. axonopodis pv. glycines KU-P-SW005 was shown to significantly decrease cellulase, pectate lyase, and polysaccharide production. The production of these proteins in the ompA mutant was approximately five times lower than that of the wildtype. The ompA mutant also exhibited modified biofilm development. More importantly, the mutant reduced disease severity to the soybean. Ten days after inoculation, the virulence rating of the susceptible soybean cv. SJ4 inoculated with the ompA mutant was 11.23%, compared with 87.98% for the complemented ompA mutant. Production of cellulase, pectate lyase, polysaccharide was restored, biofilm, and pustule numbers were restored in the complemented ompA mutant that did not differ from the wild type. Taken together, these data suggest that OmpA-mediated invasion plays an important role in protein secretion during pathogenesis to soybean.

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Acknowledgements

This work was supported by Thailand Research Fund and Thammasat University under the research grant for new scholars [Grant No. MRG5580202, 2012]. We thank S. Prathuangwong for kind recommendations. The authors wish to express special thanks to the Central Scientific Instrument Center (CSIC), Faculty of Science and Technology, Thammasat for providing scientific instrument support.

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  1. Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand

    Dusit Athinuwat

  2. School of Science, Mae Fah Luang University, Chaing Rai, 57100, Thailand

    Siraprapa Brooks

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  1. Dusit Athinuwat
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Correspondence to Dusit Athinuwat.

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Athinuwat, D., Brooks, S. The OmpA Gene of Xanthomonas axonopodis pv. glycines is Involved in Pathogenesis of Pustule Disease on Soybean. Curr Microbiol 76, 879–887 (2019). https://doi.org/10.1007/s00284-019-01702-y

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  • DOI: https://doi.org/10.1007/s00284-019-01702-y