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Enhancement the Cellulase Activity Induced by Endophytic Bacteria Using Calcium Nanoparticles

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Abstract

The huge applications of cellulosic and lignocellulosic materials in the various fields of life lead to accumulation of its wastes that became one of the major sources of environmental pollution. In this study, a Gram-positive cellulose-decomposing endophytic bacterium (Chi-04) was isolated from medicinal plant Chiliadenus montanus which inhabitant Saint Catherine (Sinai) region in Egypt. The bacterial strain was identified based on the sequence analysis of 16S rRNA genes as Lysinibacillus xylanilyticus. This isolate was capable of degrading 58% of cellulosic filter paper (100 g/l) within 15 days of incubation. The soluble and reduced sugars were spectrophotometrically determined as cellulose decomposition metabolites. The bacterial isolate exhibited an obvious activity toward cellulase enzyme production. The maximum cellulase activity (0.18 U/min) was detected after 12 days of incubation while the maximum release of soluble sugars (11.85 mg/ml) was detected after 15 days of incubation. CaCl2 nanoparticles (100 nm) were chemically prepared to enhance the activity of the enzyme. The optimum concentration of CaCl2 nanoparticles that showed the highest activity of cellulase (0.3 mg/ml reduced sugar) was 0.6%. The bacterial isolates showed potential convert of cellulose into reducing sugars which could be used in several applications.

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References

  1. Tomme P, Warren R, Gilkes N (1995) Cellulose hydrolysis by bacteria and fungi. Adv Microb Physiol 37:1–81

    Article  CAS  PubMed  Google Scholar 

  2. Ghio S, Di Lorenzo GS, Lia V, Talia P, Cataldi A, Grasso D, Campos E (2012) Isolation of Paenibacillus sp. and Variovorax sp. strains from decaying woods and characterization of their potential for cellulose deconstruction. Int J Biochem Mol Biol 3:352

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Acharya A, Joshi D, Shrestha K, Bhatta D (2012) Isolation and screening of thermophilic cellulolytic bacteria from compost piles. Sci World 10:43–46

    Article  CAS  Google Scholar 

  4. Trujillo-Cabrera Y, Ponce-Mendoza A, Vásquez-Murrieta MS, Rivera-Orduña FN, Wang ET (2013) Diverse cellulolytic bacteria isolated from the high humus, alkaline-saline chinampa soils. Ann Microbiol 63:779–792

    Article  CAS  Google Scholar 

  5. Gautam S, Bundela P, Pandey A, Khan J, Awasthi M, Sarsaiya S (2011) Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnol Res Int 2011:810425

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Murray WD, Sowden L, Colvin JR (1984) Bacteroides cellulosolvens sp. nov., a cellulolytic species from sewage sludge. Int J Syst Evol Microbiol 34:185–187

    Google Scholar 

  7. Jyotsna KP, Vijayalakshmi K, Prasanna N, Shaheen S (2010) Isolation, characterization of cellulase producing Lysinibacillus sphaericus MTCC No. 9468 from gut of Eisenia foetida. Bioscan 6(2):325–327

    Google Scholar 

  8. Prem Anand AA, Vennison SJ, Sankar SG, Gilwax Prabhu DI, Vasan PT, Raghuraman T, Jerome Geoffrey C, Vendan SE (2010) Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. J Insect Sci 10:107

    Google Scholar 

  9. Bhat K, Maheshwari R (1987) Sporotrichum thermophile growth, cellulose degradation, and cellulase activity. Appl Environ Microbiol 53:2175–2182

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Bui HB (2014) Isolation of cellulolytic bacteria, including actinomycetes, from coffee exocarps in coffee-producing areas in Vietnam. Int J Recycl Org Waste Agric 3:48

    Article  Google Scholar 

  11. Shin CS, Lee JP, Lee JS, Park SC (2000) Enzyme production of Trichoderma reesei Rut C-30 on various lignocellulosic substrates. In: Twenty-first symposium on biotechnology for fuels and chemicals. Springer, New York, pp. 237–245

    Chapter  Google Scholar 

  12. Gupta P, Samant K, Sahu A (2012) Isolation of cellulose-degrading bacteria and determination of their cellulolytic potential. Int J Microbiol 2012:578925

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Hussain AA, Abdel-Salam MS, Abo-Ghalia HH, Hegazy WK, Hafez SS (2017) Optimization and molecular identification of novel cellulose degrading bacteria isolated from Egyptian environment. J Genet Eng Biotechnol 15:77–85

    Article  PubMed  PubMed Central  Google Scholar 

  14. Nakamura K, Kitamura K (1982) Isolation and identification of crystalline cellulose hydrolyzing bacterium and its enzymatic properties. J Ferment Technol 60:343–348

    CAS  Google Scholar 

  15. Li L, Taghavi S, McCorkle SM, Zhang YB, Blewitt MG, Brunecky R, Adney WS, Himmel ME, Brumm P, Drinkwater C (2011) Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases. Biotechnol Biofuels 4:23

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Sukharnikov LO, Cantwell BJ, Podar M, Zhulin IB (2011) Cellulases: ambiguous nonhomologous enzymes in a genomic perspective. Trends Biotechnol 29:473–479

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Liang Y, Yesuf J, Schmitt S, Bender K, Bozzola J (2009) Study of cellulases from a newly isolated thermophilic and cellulolytic Brevibacillus sp. strain JXL. J Ind Microbiol Biotechnol 36:961–970

    Article  CAS  PubMed  Google Scholar 

  18. Criquet S (2002) Measurement and characterization of cellulase activity in sclerophyllous forest litter. J Microbiol Methods 50:165–173

    Article  CAS  PubMed  Google Scholar 

  19. Farnet A, Qasemian L, Guiral D, Ferré E (2010) A modified method based on arsenomolybdate complex to quantify cellulase activities: application to litters. Pedobiologia 53:159–160

    Article  Google Scholar 

  20. Yu X, Liu Y, Cui Y, Cheng Q, Zhang Z, Lu JH, Meng Q, Teng L, Ren X (2016) Measurement of filter paper activities of cellulase with microplate-based assay. Saudi J Biol Sci 23:S93–S98

    Article  CAS  PubMed  Google Scholar 

  21. Zhang YHP, Himmel ME, Mielenz JR (2006) Outlook for cellulase improvement: screening and selection strategies. Biotechnol Adv 24:452–481

    Article  CAS  Google Scholar 

  22. Ghose T (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268

    Article  CAS  Google Scholar 

  23. Butt MA, Ahmad M, Fatima A, Sultana S, Zafar M, Yaseen G, Ashraf MA, Shinwari ZK, Kayani S (2015) Ethnomedicinal uses of plants for the treatment of snake and scorpion bite in Northern Pakistan. J Ethnopharmacol 168:164–181

    Article  CAS  PubMed  Google Scholar 

  24. Hankin L, Anagnostakis SL (1977) Solid media containing carboxymethylcellulose to detect Cx cellulase activity of micro-organisms. Microbiol 98:109–115

    CAS  Google Scholar 

  25. Boulos L (1983) Medicinal plants of North Africa. Reference Publications, Algonac

    Google Scholar 

  26. Tackholm V (1974) Student’s flora of Egypt. University Press, Cairo

    Google Scholar 

  27. Hussein MA (2011) Anti-obesity, antiatherogenic, anti-diabetic and antioxidant activities of J. montana ethanolic formulation in obese diabetic rats fed high-fat diet. Free Rad Antioxid 1:49–60

    Article  CAS  Google Scholar 

  28. Al-Howiriny TA, Al-Rehaily AJ, Pols JR, Porter JR, Mossa JS, Ahmed B (2005) Three new diterpenes and the biological activity of different extracts of Jasonia montana. Nat Prod Res 19:253–265

    Article  CAS  PubMed  Google Scholar 

  29. Carder JH (1986) Detection and quantitation of cellulase by Congo red staining of substrates in a cup-plate diffusion assay. Anal Biochem 153:75–79

    Article  CAS  PubMed  Google Scholar 

  30. Mawad AM, Hesham AEL, Mostafa YM, Shoriet A (2016) Pyrene degrading Achromobacter denitrificans ASU-035: growth rate, enzymes activity, and cell surface properties. Rend Lincei 27:557–563

    Article  Google Scholar 

  31. Huson DH, Bryant D (2005) Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23:254–267

    Article  CAS  PubMed  Google Scholar 

  32. Yemm E, Willis A (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  34. Ali SM, Yousef NM, Nafady NA (2015) Application of biosynthesized silver nanoparticles for the control of land snail Eobania vermiculata and some plant pathogenic fungi. J Nanomater 2015:3

    Google Scholar 

  35. Mekkawy AI, El-Mokhtar MA, Nafady NA, Yousef N, Hamad MA, El-Shanawany SM, Ibrahim EH, Elsabahy M (2017) In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels. Int J Nanomed 12:759

    Article  CAS  Google Scholar 

  36. Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780

    CAS  PubMed  PubMed Central  Google Scholar 

  37. Patagundi BI, Shivasharan C, Kaliwal B (2014) Isolation and characterization of cellulase producing bacteria from soilInt. Int J Curr Microbiol Appl Sci 3:59–69

    Google Scholar 

  38. Lee CS, Jung YT, Park S, Oh TK, Yoon JH (2010) Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus. Int J Syst Evol Microbiol 60:281–286

    Article  CAS  PubMed  Google Scholar 

  39. Nongkhlaw W, Mary F, Joshi S (2014) Epiphytic and endophytic bacteria that promote growth of ethnomedicinal plants in the subtropical forests of Meghalaya, India. Rev Biol Trop 62:1295–1308

    Article  PubMed  Google Scholar 

  40. Ekperigin M (2007) Preliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp. Afr J Biotechnol 6:28–33

    CAS  Google Scholar 

  41. Khianngam S, Pootaeng-on Y, Techakriengkrai T, Tanasupawat S (2014) Screening and identification of cellulase producing bacteria isolated from oil palm meal. J Appl Pharm Sci 4:90

    CAS  Google Scholar 

  42. Gupta P, Parkhey P (2015) Design of a single chambered microbial electrolytic cell reactor for production of biohydrogen from rice straw hydrolysate. Biotechnol Lett 37:1213–1219

    Article  CAS  PubMed  Google Scholar 

  43. Ariffin H, Abdullah N, Umi Kalsom M, Shirai Y, Hassan M (2006) Production and characterization of cellulase by Bacillus pumilus EB3. Int J Eng Technol 3:47–53

    Google Scholar 

  44. Vaheri M (1982) Acidic degradation products of cellulose during enzymatic hydrolysis by Trididerma reesei. J Appl Biochem 4:153–160

    CAS  Google Scholar 

  45. Yousef AE (2017) Antimicrobial effects of calcium oxide nanoparticles and some spices in minced meat. ARC J Anim Vet Sci 3:7

    Google Scholar 

  46. McGavin M, Forsberg C (1988) Isolation and characterization of endoglucanases 1 and 2 from Bacteroides succinogenes S85. J Bacteriol 170:2914–2922

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. McGavin M, Lam J, Forsberg CW (1990) Regulation and distribution of Fibrobacter succinogenes subsp. succinogenes S85 endoglucanases. Appl Environ microbiol 56:1235–1244

    CAS  PubMed  PubMed Central  Google Scholar 

  48. Mitsumori M, Minato H (2000) Identification of the cellulose-binding domain of Fibrobacter succinogenes endoglucanase. FEMS Microbiol Lett 183:99–103

    Article  CAS  PubMed  Google Scholar 

  49. Mitsumori M, Xu L, Kajikawa H, Kurihara M (2002) Properties of cellulose-binding modules in endoglucanase F from Fibrobacter succinogenes S85 by means of surface plasmon resonance. EMS Microbiol Lett 214:277–281

    Article  CAS  Google Scholar 

  50. Nomura H, Shiina T (2014) Calcium signaling in plant endosymbiotic organelles: mechanism and role in physiology. Mol Plant 7:1094–1104

    Article  CAS  PubMed  Google Scholar 

  51. Morales M, Dehority B (2009) Ionized calcium requirement of rumen cellulolytic bacteria. J Dairy Sci 92:5079–5091

    Article  CAS  PubMed  Google Scholar 

  52. Gong J, Forsberg CW (1989) Factors affecting adhesion of Fibrobacter succinogenes subsp. succinogenes S85 and adherence-defective mutants to cellulose. Appl Environ Microbiol 55:3039–3044

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors would thank Prof. Abd El-aziz A. Said, Chemistry Department, Faculty of Science, Assiut University, Egypt for kindly providing CaCl2 nanoparticles.

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  1. Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt

    Naiema Yousef, Asmaa Mawad & Amany Abeed

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  1. Naiema Yousef
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  2. Asmaa Mawad
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  3. Amany Abeed
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Correspondence to Asmaa Mawad.

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Yousef, N., Mawad, A. & Abeed, A. Enhancement the Cellulase Activity Induced by Endophytic Bacteria Using Calcium Nanoparticles. Curr Microbiol 76, 346–354 (2019). https://doi.org/10.1007/s00284-018-1614-x

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