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Complete Genome Sequence of Saccharospirillum mangrovi HK-33T Sheds Light on the Ecological Role of a Bacterium in Mangrove Sediment Environment

Abstract

We present the genome sequence of Saccharospirillum mangrovi HK-33T, isolated from a mangrove sediment sample in Haikou, China. The complete genome of S. mangrovi HK-33T consisted of a single-circular chromosome with the size of 3,686,911 bp as well as an average G + C content of 57.37%, and contained 3,383 protein-coding genes, 4 operons of 16S-23S-5S rRNA genes, and 52 tRNA genes. Genomic annotation indicated that the genome of S. mangrovi HK-33T had many genes related to oligosaccharide and polysaccharide degradation and utilization of polyhydroxyalkanoate. For nitrogen cycle, genes encoding nitrate and nitrite reductase, glutamate dehydrogenase, glutamate synthase, and glutamine synthetase could be found. For phosphorus cycle, genes related to polyphosphate kinases (ppk1 and ppk2), the high-affinity phosphate-specific transport (Pst) system, and the low-affinity inorganic phosphate transporter (pitA) were predicted. For sulfur cycle, cysteine synthase and type III acyl coenzyme A transferase (dddD) coding genes were searched out. This study provides evidence about carbon, nitrogen, phosphorus, and sulfur metabolic patterns of S. mangrovi HK-33T and broadens our understandings about ecological roles of this bacterium in the mangrove sediment environment.

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Acknowledgements

This work was supported by the Natural Science Foundation of Zhejiang Province of China (LQ18C010001, LH19H300001), the Natural Science Foundation of China (41776168), the Natural Science Foundation of Ningbo City (2018A610303), Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources (NBHY-2017-P2), Ningbo Sci. & Tech. Projects for Common Wealth (2017C10016), the 111 project (D16013), the Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund, the K.C. Wong Magna Fund in Ningbo University.

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Correspondence to Slava S. Epstein or Shan He.

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Nucleotide sequence accession number: The complete genome sequence of S. mangrovi HK-33T was deposited in DDBJ/EMBJ/GenBank under the accession number CP031415.

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Zhang, W., Zhou, X., Yuan, Y. et al. Complete Genome Sequence of Saccharospirillum mangrovi HK-33T Sheds Light on the Ecological Role of a Bacterium in Mangrove Sediment Environment. Curr Microbiol 76, 231–236 (2019). https://doi.org/10.1007/s00284-018-1600-3

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  • DOI: https://doi.org/10.1007/s00284-018-1600-3