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Hymenobacter pomorum sp. nov., Isolated from Apple Orchard Soil

  • Leonid N. Ten
  • Weilan Li
  • Seung-Yeol Lee
  • In-Kyu Kang
  • Young-Je Cho
  • Myung Kyum Kim
  • Hee-Young Jung
Article
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Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated 9-2-1-1T, was isolated from apple orchard soil in Daegu, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that the isolate belongs to the family Cytophagaceae, Bacteroidetes and it is most closely related to Hymenobacter metalli A2-91T (97.8% similarity) and Hymenobacter marinus KJ035T (96.6%). Growth of strain 9-2-1-1T was observed at 4–30 °C, pH 6–8, and in the presence of 0–1.0% NaCl. The G+C content of the genomic DNA was 62.0 mol%. The predominant respiratory quinone of the isolate was MK-7; the major fatty acids were C15:0 iso (29.3%), C16:1 ω5c (15.4%), C15:0 anteiso (12.5%), summed feature 3 (C16:1 ω7c/C16:1 ω6c; 12.3%), and C16:0 (10.6%); and the major polar lipid was phosphatidylethanolamine. The phenotypic and chemotaxonomic data supported the affiliation of strain 9-2-1-1T with the genus Hymenobacter. However, the DNA–DNA relatedness between the isolate and H. metalli and H. marinus were 31.3% and 24.7%, respectively. The DNA–DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 9-2-1-1T is the representative of a novel species in the genus Hymenobacter, for which the name Hymenobacter pomorum sp. nov. is proposed. The type strain is 9-2-1-1T (=KCTC 52740T = JCM 32193T).

Notes

Acknowledgements

This work was supported by the Brain Pool Program (Grant No. 2018H1D3A2065415) through the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea.

Compliance with Ethical Standards

Conflict of interest

No conflict of interest is declared.

Supplementary material

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Supplementary material 1 (DOCX 1666 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Leonid N. Ten
    • 1
  • Weilan Li
    • 1
  • Seung-Yeol Lee
    • 1
    • 5
  • In-Kyu Kang
    • 2
  • Young-Je Cho
    • 3
  • Myung Kyum Kim
    • 4
  • Hee-Young Jung
    • 1
    • 5
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Horticultural ScienceKyungpook National UniversityDaeguRepublic of Korea
  3. 3.School of Food Science and Biotechnology/Food and Bio-Industry Research InstituteKyungpook National UniversityDaeguRepublic of Korea
  4. 4.Department of Bio and Environmental TechnologySeoul Women’s UniversitySeoulRepublic of Korea
  5. 5.Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea

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