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Current Microbiology

, Volume 75, Issue 11, pp 1530–1536 | Cite as

Brevundimonas mongoliensis sp. nov., A Novel Psychrotolerant Bacterium Isolated from Oil-Contaminated Soil

  • Dhiraj Kumar Chaudhary
  • Jaisoo KimEmail author
Article

Abstract

Two yellow-coloured, Gram-stain-negative, motile, and rod-shaped bacteria, designated strains R-10-10T and R-10-15 were isolated from oil-contaminated soil. Both strains were able to grow at 4–40 °C, pH 5.5–10.5, and 0–4% (w/v) NaCl concentration. These strains were taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, both strains, R-10-10T and R-10-15, could be affiliated to the genus Brevundimonas and shared highest sequence similarity with Brevundimonas staleyi FWC43T (98.8%), Brevundimonas bullata TK0051T (98.6%), and Brevundimonas subvibrioides CB81T (98.3%). The pairwise sequence similarity between strains R-10-10T and R-10-15 was 99.9%. Both strains R-10-10T and R-10-15 contained phosphatidylglycerol, diphosphatidylglycerol, and four unidentified glycolipids as major polar lipids; ubiquinone-10 as sole respiratory quinone; and summed feature 8 (C18:1ω7c and/or C18:1ω6c), C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and C18:1ω9c as major fatty acids. The genomic DNA G+C content values of strains R-10-10T and R-10-15 were 67.1 and 66.9 mol%, respectively. The DNA–DNA relatedness between R-10-10T and R-10-15 was higher than 70% but the values were less than 55% with closely related reference type strains. The morphological, physiological, chemotaxonomic, and phylogenetic data clearly distinguished strain R-10-10T from its closest phylogenetic neighbors. Thus, strain R-10-10T is considered to represent a novel species of the genus Brevundimonas, for which the name Brevundimonas mongoliensis sp. nov. is proposed. The type strain is R-10-10T (=KEMB 9005-696T = KACC 19387T = JCM 32172T), and strain R-10-15 is considered as an additional strain of the novel species.

Abbreviations

KEMB

Korea Environmental Microorganisms Bank

KACC

Korean agricultural culture collection

JCM

Japan Collection of Microorganisms

DSM

Deutsche Sammlung von Mikroorganismen

Notes

Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982).

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest.

Research Involving Human Participants

This study does not describe any experimental work related to human.

Supplementary material

284_2018_1555_MOESM1_ESM.docx (81 kb)
Supplementary material 1 (DOCX 81 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Life Science, College of Natural SciencesKyonggi UniversitySuwonSouth Korea

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