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Implementing Electric Potential Difference as a New Practical Parameter for Rapid and Specific Measurement of Minimum Inhibitory Concentration of Antibiotics

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Abstract

New methods to determine antimicrobial susceptibility of bacterial pathogens especially the minimum inhibitory concentration (MIC) of antibiotics have great importance in pharmaceutical industry and treatment procedures. In the present study, the MIC of several antibiotics was determined against some pathogenic bacteria using macrodilution test. In order to accelerate and increase the efficiency of culture-based method to determine antimicrobial susceptibility, the possible relationship between the changes in some physico-chemical parameters including conductivity, electrical potential difference (EPD), pH and total number of test strains was investigated during the logarithmic phase of bacterial growth in presence of antibiotics. The correlation between changes in these physico-chemical parameters and growth of bacteria was statistically evaluated using linear and non-linear regression models. Finally, the calculated MIC values in new proposed method were compared with the MIC derived from macrodilution test. The results represent significant association between the changes in EPD and pH values and growth of the tested bacteria during the exponential phase of bacterial growth. It has been assumed that the proliferation of bacteria can cause the significant changes in EPD values. The MIC values in both conventional and new method were consistent to each other. In conclusion, cost and time effective antimicrobial susceptibility test can be developed based on monitoring the changes in EPD values. The new proposed strategy also can be used in high throughput screening of biocompounds for their antimicrobial activity in a relatively shorter time (6–8 h) in comparison with the conventional methods.

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Acknowledgements

This research project was financially sponsored by Iran National Science Foundation by (Grant No. 93027010). The facilities were provided by Microbial Technology and Product Research Center, University of Tehran.

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Authors and Affiliations

  1. Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Nasrin Mobasheri & Javad Hamedi

  2. Microbial Technology and Products (MTP) Research Center, University of Tehran, Tehran, Iran

    Nasrin Mobasheri & Javad Hamedi

  3. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Mehrdad Karimi

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  1. Nasrin Mobasheri
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  2. Mehrdad Karimi
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Correspondence to Javad Hamedi.

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Mobasheri, N., Karimi, M. & Hamedi, J. Implementing Electric Potential Difference as a New Practical Parameter for Rapid and Specific Measurement of Minimum Inhibitory Concentration of Antibiotics. Curr Microbiol 75, 1290–1298 (2018). https://doi.org/10.1007/s00284-018-1523-z

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