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Pseudomonas pharmafabricae sp. nov., Isolated From Pharmaceutical Wastewater

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Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-ZT, was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4–42 °C (optimum, 35 °C), pH 5.0–9.0 (optimum, 6.0) and 0–5.0% (w/v) NaCl (optimum, 2.0%). Based on multilocus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD, the strain ZYSR67-ZT formed a distinct phylogenetic group in the genus Pseudomonas. The average nucleotide identity values between strain ZYSR67-ZT and the closely related 10 type strains of the Pseudomonas species were 75.8–78.6%. The in silico DNA–DNA hybridization values indicated that strain ZYSR67-ZT and the type strains of the Pseudomonas shared 21.4–23.1% DNA relatedness. The predominant isoprenoid quinone system was ubiquinone-9 while ubiquinone-8 was present in trace amounts. The major fatty acids (> 10%) identified were C12:0, C16:0, C18:1 ω7c and summed features 3 (C16:1 ω7c and/or iso-C15:0 2OH). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 62.6 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain ZYSR67-ZT was proposed to represent a novel species of the genus Pseudomonas, named Pseudomonas pharmafabricae sp. nov.. The type strain is ZYSR67-ZT (= CGMCC 1.15498T = JCM 31306T).

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Acknowledgements

This work was supported by grants from the Zhejiang Provincial Public Welfare Technology Applied Research Project (2017C33030) and (2017C33046).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Xiao-Yun Yu & Min Wu

  2. College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China

    Jing-Yu Zhai, Jie-Ying Shi & Lin-Xian Ding

  3. Zhejiang University of Water Resources and Electric Power, Hangzhou, 310018, People’s Republic of China

    Chen Wu

  4. Ocean College, Zhejiang University, Zhoushan, 316000, People’s Republic of China

    Chong-Ya Zhang

Authors
  1. Xiao-Yun Yu
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  2. Jing-Yu Zhai
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  5. Jie-Ying Shi
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  6. Lin-Xian Ding
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Correspondence to Lin-Xian Ding or Min Wu.

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Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene, gyrB, rpoB and rpoD sequences of strain ZYSR67-ZT are KX910087, KY887561, KY887563 and KY887562, respectively. WGS whole-genome project accession number for strain ZYSR67-ZT is PIYS00000000. The Digital Protologue database Taxon Number for strain ZYSR67-ZT is TA00200.

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Yu, XY., Zhai, JY., Wu, C. et al. Pseudomonas pharmafabricae sp. nov., Isolated From Pharmaceutical Wastewater. Curr Microbiol 75, 1119–1125 (2018). https://doi.org/10.1007/s00284-018-1495-z

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  • DOI: https://doi.org/10.1007/s00284-018-1495-z