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A New Approach of Rpf Addition to Explore Bacterial Consortium for Enhanced Phenol Degradation Under High Salinity Conditions

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Abstract

Only a small fraction of salt-tolerant phenol-degrading bacteria can be isolated by conventional plate separation methods, because most bacteria in nature are in a viable but non-culturable (VBNC) state. The aims of this study were to screen out more effective functional bacteria using resuscitation-promoting factor (Rpf), and to determine whether a mixed bacterial consortium possesses better phenol-degrading capabilities under high salinity conditions. The results indicated that three strains unique to treatment group with Rpf addition were obtained. A mixed bacterial consortium consisting of two high-efficient strains which belonged to genera Bacillus and Corynebacterium was capable of utilizing phenol as a sole source of carbon at high salinity. Complete degradation of 100 mg/L phenol at 2% NaCl concentration was achieved within 8 h. This study provides new insights into resuscitation of VBNC bacteria for enhanced treatment of phenol-laden saline wastewater.

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Acknowledgements

We gratefully acknowledge the financial supports provided by the Special Fund for the Zhejiang Research Academy of Environmental Science (Grant No. 2017F30030), the National Natural Science Foundation of China (Grant No. 41701354), the Natural Science Foundation of Zhejiang Province of China (Grant No. LQ17D010002), and the Research Fund for the Doctoral Research of Key University (Grant No. ZZ323205020516001108).

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Author notes

  1. Ziqiao Li and Yunge Zhang have contributed equally to this paper.

Authors and Affiliations

  1. College of Geography and Environmental Science, Zhejiang Normal University, Yingbin Road 688#, Jinhua, 321004, China

    Ziqiao Li, Yunge Zhang, Yuyang Wang, Hongjun Lin & Xiaomei Su

  2. Environmental Science Research and Design Institute of Zhejiang Province, Hangzhou, 310007, China

    Rongwu Mei & Yu Zhang

  3. Department of Meteorology, COMSATS Institute of Information Technology, Islamabad, 44000, Pakistan

    Muhammad Zaffar Hashmi

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  1. Ziqiao Li
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  2. Yunge Zhang
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Correspondence to Xiaomei Su.

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Li, Z., Zhang, Y., Wang, Y. et al. A New Approach of Rpf Addition to Explore Bacterial Consortium for Enhanced Phenol Degradation Under High Salinity Conditions. Curr Microbiol 75, 1046–1054 (2018). https://doi.org/10.1007/s00284-018-1489-x

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