Current Microbiology

, Volume 75, Issue 8, pp 1011–1015 | Cite as

The Draft Genome Sequence of a Novel High-Efficient Butanol-Producing Bacterium Clostridium Diolis Strain WST

  • Chaoyang Chen
  • Chongran Sun
  • Yi-Rui Wu


A wild-type solventogenic strain Clostridium diolis WST, isolated from mangrove sediments, was characterized to produce high amount of butanol and acetone with negligible level of ethanol and acids from glucose via a unique acetone-butanol (AB) fermentation pathway. Through the genomic sequencing, the assembled draft genome of strain WST is calculated to be 5.85 Mb with a GC content of 29.69% and contains 5263 genes that contribute to the annotation of 5049 protein-coding sequences. Within these annotated genes, the butanol dehydrogenase gene (bdh) was determined to be in a higher amount from strain WST compared to other Clostridial strains, which is positively related to its high-efficient production of butanol. Therefore, we present a draft genome sequence analysis of strain WST in this article that should facilitate to further understand the solventogenic mechanism of this special microorganism.



This work was financially supported the “Sail Plan” Program for the Introduction of Outstanding Talents of Guangdong Province of China (No. 14600601), the Major University Research Foundation of Guangdong Province of China (No. 2015KQNCX041), the Start-Up Funding of Shantou University (No. NTF15007), the International Cooperation Research Project of Shantou University (No. NC2017001) and the Foundation of Guangdong Provincial Key Laboratory of Marine Biotechnology (No. GPKLMB201702).

Compliance with Ethical Standards

Conflict of interest

The authors have declared there was no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiologyShantou UniversityShantouChina
  2. 2.STU-UNIVPM Joint Algal Research CenterShantou UniversityShantouChina
  3. 3.Guangdong Provincial Key Laboratory of Marine BiotechnologyShantou UniversityShantouChina

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