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Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen

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Abstract

The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.

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Funding

Funding was provided by Universidad Nacional de La Plata (Grant No. Subsidio Incentivos 2015), Comision de Investigaciones de la Provincia de Buenos Aires (Subsidio 2015) and Jefatura de Gabinete de Ministros de la Nacion (Grant No. BECAR2014).

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Authors and Affiliations

  1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Silvina M. Y. López

  2. Centro de Investigaciones de Fitopatología (CIDEFI - CICBA-UNLP), Buenos Aires, Argentina

    Silvina M. Y. López, Mario E. E. Franco & Pedro A. Balatti

  3. Grupo de Ecología Genética de la Rizosfera, Dpto. Microbiología y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Ma. Dolores Molina Sánchez & Nicolás Toro García

  4. Cátedra de Microbiología Agrícola, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Calle 60 y 119, La Plata, 1900, Argentina

    Graciela N. Pastorino & Pedro A. Balatti

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  1. Silvina M. Y. López
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  2. Ma. Dolores Molina Sánchez
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  3. Graciela N. Pastorino
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Correspondence to Pedro A. Balatti.

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López, S.M.Y., Sánchez, M.D.M., Pastorino, G.N. et al. Nodulation and Delayed Nodule Senescence: Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen. Curr Microbiol 75, 997–1005 (2018). https://doi.org/10.1007/s00284-018-1478-0

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