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Agromyces mangrovi sp. nov., a Novel Actinobacterium Isolated from Mangrove Soil

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Abstract

A novel, Gram-stain-positive, microaerophilic to aerobic, non-endospore-forming, no-motile and rod-shaped bacterium designated Q14T was isolated from mangrove soil samples collected on chengmai, Hainan province, China. Strain Q14T was able to grow at 10–40 °C (optimum 30 °C), pH 5.5–10.0 (optimum 6.5-8.0) and with 0.5–6% (w/v) NaCl (optimum 1%). The genomic DNA G+C content was 70.1%. The chemotaxonomic analysis showed that the predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C17:0 and anteiso-C17:0. The major polar lipids of strain Q14T were diphosphatidylglycerol, phosphatidylglycerol and one glycolipid. The strain Q14T contained 2,4-diaminobutylic acid (A2bu), alanine acid, glutamic acid and glycine in the peptidoglycans. The phylogenetic analysis and DNA–DNA hybridization, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q14T as a novel species of the genus Agromyces, for which the name Agromyces mangrovi sp. nov. is proposed. The type strain is Q14T (= MCCC 1K03191T = KCTC 39814T).

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Acknowledgements

We thank staff of Pharmaceutical institute of hainan province, for their provision of mangrove soil samples. This study was supported by Grants from the National Natural Science Foundation of China (31470005).

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Correspondence to Xiaopin Li or Min Wu.

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Ruijun Wang and Can Chen have contributed equally to this study and are joint first authors.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Q14T is KX170835 and the DPD number is TA00333.

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Wang, R., Chen, C., Su, Y. et al. Agromyces mangrovi sp. nov., a Novel Actinobacterium Isolated from Mangrove Soil. Curr Microbiol 75, 1055–1061 (2018). https://doi.org/10.1007/s00284-018-1476-2

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  • DOI: https://doi.org/10.1007/s00284-018-1476-2

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